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searching for Chain termination 68 found (94 total)

Translation (biology) (3,974 words) [view diff] no match in snippet view article

codon to continue the process (translocation), creating an amino acid chain. Termination: When a stop codon is reached, the ribosome releases the polypeptide

Sanger sequencing (3,257 words) [view diff] no match in snippet view article find links to article

Prevention's (CDC) CaliciNet surveillance network. The classical chain-termination method requires a single-stranded DNA template, a DNA primer, a DNA

Shotgun sequencing (2,743 words) [view diff] no match in snippet view article find links to article

the rapidly expanding, quasi-random shot grouping of a shotgun. The chain-termination method of DNA sequencing ("Sanger sequencing") can only be used for

Puromycin (829 words) [view diff] exact match in snippet view article find links to article

an antibiotic protein synthesis inhibitor which causes premature chain termination during translation. Puromycin is an aminonucleoside antibiotic, derived

Nonsense mutation (2,870 words) [view diff] exact match in snippet view article find links to article

premature termination of polypeptide chains; they are also called chain termination mutations. Missense mutations differ from nonsense mutations since

Massive parallel sequencing (3,019 words) [view diff] no match in snippet view article find links to article

first-generation sequencing—which is based on electrophoretic separation of chain-termination products produced in individual sequencing reactions. This methodology

Telbivudine (648 words) [view diff] exact match in snippet view article find links to article

Telbivudine impairs hepatitis B virus (HBV) DNA replication by leading to chain termination. It differs from the natural nucleotide only with respect to the location

DNA sequencing (14,413 words) [view diff] no match in snippet view article find links to article

sequence may be inferred. This method is mostly obsolete as of 2023. The chain-termination method developed by Frederick Sanger and coworkers in 1977 soon became

Ziegler–Natta catalyst (1,843 words) [view diff] exact match in snippet view article find links to article

occasion, the polymer chain is disengaged from the active centers in the chain termination reaction. Several pathways exist for termination: Cp2+Zr−(CH2−CHR)n−CH3

Primer walking (1,168 words) [view diff] exact match in snippet view article find links to article

fragments cannot be sequenced in a single sequence read using the chain termination method. This method works by dividing the long sequence into several

Mitochondrial translational release factor 1 (613 words) [view diff] exact match in snippet view article find links to article

directs the termination of translation in response to the peptide chain termination codons. Initially thought to have a role in the termination of mitochondria

Valganciclovir (1,265 words) [view diff] exact match in snippet view article find links to article

incorporated into viral DNA due to its structure similarity to dGTP, chain termination occurs once a single nucleotide is added to the distal hydroxyl group

Photochlorination (2,997 words) [view diff] exact match in snippet view article find links to article

\mathrm {Cl{\cdot }+{\cdot }Cl\longrightarrow Cl_{2}\quad (chain\ termination)} } Chain termination occurs by recombination of chlorine atoms. Impurities such

Genomics (7,518 words) [view diff] no match in snippet view article find links to article

genome. The refinement of the Plus and Minus method resulted in the chain-termination, or Sanger method (see below), which formed the basis of the techniques

Splice site mutation (1,719 words) [view diff] exact match in snippet view article find links to article

59 base-pair insertion and chain termination. The four families with both breast and ovarian cancer had chain termination mutations in the N-terminal

Sequencing (1,697 words) [view diff] exact match in snippet view article find links to article

fragment. So far, most DNA sequencing has been performed using the chain termination method developed by Frederick Sanger. This technique uses sequence-specific

DNA sequencer (3,850 words) [view diff] exact match in snippet view article find links to article

specific bases whereas Sanger's technique is based on dideoxynucleotide chain termination. The Sanger method became popular due to its increased efficiency

Autoxidation (1,494 words) [view diff] exact match in snippet view article find links to article

accelerating reaction. As the concentration of radicals increases chain termination reactions become more important, these reduce the number of radicals

Reaction mechanism (1,623 words) [view diff] exact match in snippet view article find links to article

another chain carrier, but the product concentration is reduced. Chain termination: radicals combine and the chain carriers are lost. Inhibition: chain

Release factor (1,728 words) [view diff] exact match in snippet view article find links to article

is mediated by ABCE1. Capecchi MR (September 1967). "Polypeptide chain termination in vitro: isolation of a release factor". Proceedings of the National

CRYBB2 (983 words) [view diff] exact match in snippet view article find links to article

1997). "Autosomal dominant cerulean cataract is associated with a chain termination mutation in the human beta-crystallin gene CRYBB2". Hum Mol Genet

Autoacceleration (611 words) [view diff] exact match in snippet view article find links to article

previously formed polymer molecules increases. Before autoacceleration, chain termination by combination of two free-radical chains is a very rapid reaction

Maxam–Gilbert sequencing (667 words) [view diff] no match in snippet view article find links to article

production of single-stranded DNA. However, with the improvement of the chain-termination method (see below), Maxam–Gilbert sequencing has fallen out of favour

Pyrosequencing (1,110 words) [view diff] exact match in snippet view article find links to article

of 300-500 nucleotides, shorter than the 800-1000 obtainable with chain termination methods (e.g. Sanger sequencing). This can make the process of genome

Directionality (molecular biology) (1,191 words) [view diff] no match in snippet view article

interrupt the replication of DNA. This technique is known as the dideoxy chain-termination method or the Sanger method, and is used to determine the order of

Primer (molecular biology) (2,112 words) [view diff] exact match in snippet view article

biological approaches involving the analysis of DNA. Both the Sanger chain termination method and the “Next-Gen” method of DNA sequencing require primers

T7 DNA polymerase (2,535 words) [view diff] no match in snippet view article find links to article

and thus, a high ratio of ddNTP to dNTP must be used for efficient chain-termination. T7 DNA polymerase discriminates against ddNTP only several fold;

Lethal allele (1,551 words) [view diff] exact match in snippet view article find links to article

produces a "nonsense codon" within a gene that causes polypeptide chain termination during translation. This finding provided insight into a significant

Genetics (10,393 words) [view diff] no match in snippet view article find links to article

at which genetic evolution occurs. One important development was chain-termination DNA sequencing in 1977 by Frederick Sanger. This technology allows

Stop codon (2,786 words) [view diff] case mismatch in snippet view article find links to article

W.; Kaplan, S. (1965). "Genetic Code: The 'Nonsense' Triplets for Chain Termination and their Suppression". Nature. 206 (4988): 994–8. Bibcode:1965Natur

Valaciclovir (1,469 words) [view diff] exact match in snippet view article find links to article

monophosphate form also incorporates into the viral DNA, resulting in chain termination. It has also been shown that the viral enzymes cannot remove aciclo-GMP

Dideoxynucleotide (1,180 words) [view diff] no match in snippet view article find links to article

DNA sequence. Thus, these molecules form the basis of the dideoxy chain-termination method of DNA sequencing, which was reported by Frederick Sanger and

Michael addition reaction (2,825 words) [view diff] exact match in snippet view article find links to article

addition. This process repeats until the reaction is quenched by chain termination. The original Michael donor can be a neutral donor such as amines

Anionic addition polymerization (1,843 words) [view diff] exact match in snippet view article find links to article

molecular weight and narrow molecular weight distribution without chain termination or transfer. Sequential monomer addition is the dominant method, also

Gemcitabine (2,417 words) [view diff] exact match in snippet view article find links to article

normal, nucleoside base to be added next to it. This leads to "masked chain termination" because gemcitabine is a "faulty" base, but due to its neighboring

Branching (polymer chemistry) (1,187 words) [view diff] exact match in snippet view article

tackle these problems. Due to their structure they have less premature chain termination and branching. IUPAC definition branching index: A parameter, g, characterizing

Tenofovir disoproxil (2,368 words) [view diff] exact match in snippet view article find links to article

residue), the active compound that inhibits reverse transcriptase via chain termination. In fasting persons, bioavailability is 25%, and highest blood plasma

Aciclovir (3,779 words) [view diff] exact match in snippet view article find links to article

monophosphate form also incorporates into the viral DNA, resulting in chain termination. Resistance to aciclovir is rare in people with healthy immune systems

Charles C. Richardson (1,954 words) [view diff] no match in snippet view article find links to article

modified DNA polymerase would be ideal for DNA sequencing by the chain-termination method. Richardson based this finding off of three main factors: high

List of experiments (2,419 words) [view diff] exact match in snippet view article find links to article

organic reactions. Frederick Sanger demonstrates the dideoxy- or chain termination method for determining DNA sequences (1975). Kary Mullis demonstrates

Gel electrophoresis (5,057 words) [view diff] exact match in snippet view article find links to article

DNA or RNA is usually done by agarose gel electrophoresis. See the "chain termination method" page for an example of a polyacrylamide DNA sequencing gel

Nylon (7,660 words) [view diff] exact match in snippet view article find links to article

to get the proportions exactly correct, and deviations can lead to chain termination at molecular weights less than a desirable 10,000 daltons (u). To

Translational bioinformatics (2,815 words) [view diff] exact match in snippet view article find links to article

2008, sequencing centers transitioned from Sanger-based (dideoxy chain termination sequencing) to 'second generation' (or 'next-generation') DNA sequencing

Daptomycin (3,758 words) [view diff] exact match in snippet view article find links to article

line has a C-A-T-TE organization. The thioesterase domain catalyzes chain termination and release of the mature lipopeptide. The molecular engineering of

Bioinformatics (8,426 words) [view diff] no match in snippet view article find links to article

method of choice for virtually all genomes sequenced (rather than chain-termination or chemical degradation methods), and genome assembly algorithms are

Biosynthesis (6,671 words) [view diff] exact match in snippet view article find links to article

for one of the 20 amino acids, while the remaining codons specify chain termination. As previously mentioned, translation occurs in three phases: initiation

Flory–Stockmayer theory (1,120 words) [view diff] exact match in snippet view article find links to article

above are satisfied under the assumptions that (1) the probability of chain termination is independent of chain length, and (2) multifunctional co-monomers

Alkane (5,927 words) [view diff] exact match in snippet view article find links to article

hydrogen from the alkane to give an alkyl radical. This reacts further. Chain termination where the radicals recombine. Experiments have shown that all halogenation

Fatty acid synthesis (4,754 words) [view diff] exact match in snippet view article find links to article

of more molecules, including "medium-chain" fatty acids via early chain termination. Once formed, the 16:0 carbon fatty acid can undergo a number of modifications

Frederick Sanger (5,320 words) [view diff] no match in snippet view article find links to article

one another. In 1977 Sanger and colleagues introduced the "dideoxy" chain-termination method for sequencing DNA molecules, also known as the "Sanger method"

Fluorescence in the life sciences (3,139 words) [view diff] exact match in snippet view article find links to article

targets within a single image. Automated sequencing of DNA by the chain termination method; each of four different chain terminating bases has its own

Pritelivir (1,008 words) [view diff] exact match in snippet view article find links to article

analogues (NA) which act via the viral DNA polymerase, causing DNA chain termination and prevention of viral replication. First-line treatment is generally

Gene (12,720 words) [view diff] no match in snippet view article find links to article

of bacteriophage MS2 coat protein. The subsequent development of chain-termination DNA sequencing in 1977 by Frederick Sanger improved the efficiency

Fluorescence (10,883 words) [view diff] exact match in snippet view article find links to article

Fluorescent glucose biosensors. Automated sequencing of DNA by the chain termination method; each of four different chain terminating bases has its own

List of MeSH codes (G06) (1,845 words) [view diff] exact match in snippet view article

chain initiation, translational MeSH G06.535.770.871.720 – peptide chain termination, translational MeSH G06.535.770.871.790 – protein modification, translational

Hofmann–Löffler reaction (5,291 words) [view diff] exact match in snippet view article find links to article

involves acceleration of the propagation steps and/or retardation of the chain termination. The influence of certain acidic solvents on the photolytic Hofmann–Löffler–Freytag

Single-molecule real-time sequencing (3,165 words) [view diff] exact match in snippet view article find links to article

that from the Sanger sequencing method based on dideoxynucleotide chain termination. The longer read length allows de novo genome sequencing and easier

Fatty acid metabolism (5,569 words) [view diff] exact match in snippet view article find links to article

of more molecules, including "medium-chain" fatty acids via early chain termination. Enzymes, acyltransferases and transacylases, incorporate fatty acids

Sofosbuvir (5,827 words) [view diff] exact match in snippet view article find links to article

Beigelman L, Deval J (July 2014). "Efficiency of incorporation and chain termination determines the inhibition potency of 2'-modified nucleotide analogs

Copper-based reversible-deactivation radical polymerization (1,639 words) [view diff] exact match in snippet view article find links to article

polymerization equilibrium (chain propagation, activation/deactivation, chain termination) and the copper conversion equilibrium (disproportionation/disproportionation)

Seafood mislabelling (7,421 words) [view diff] no match in snippet view article find links to article

Results from an automated chain-termination DNA sequencing

Mouse model of colorectal and intestinal cancer (2,526 words) [view diff] no match in snippet view article find links to article

PMID 2296722. Fodde R, Edelmann W, Yang K, et al. (1994). "A targeted chain-termination mutation in the mouse Apc gene results in multiple intestinal tumors"

List of British innovations and discoveries (13,381 words) [view diff] exact match in snippet view article find links to article

by Francis Crick and the American James Watson DNA sequencing by chain termination – Frederick Sanger Electrolysis and electrochemistry discovered :

Discovery and development of NS5A inhibitors (5,210 words) [view diff] case mismatch in snippet view article find links to article

L.; Deval, J. (14 April 2014). "Efficiency of Incorporation and Chain Termination Determines the Inhibition Potency of 2'-Modified Nucleotide Analogs

Raymond F. Schinazi (2,571 words) [view diff] exact match in snippet view article find links to article

incorporation of FTC-TP into the growing viral DNA strand results in chain termination, disrupting viral DNA synthesis. Disruption of this process results

List of English inventions and discoveries (16,528 words) [view diff] exact match in snippet view article find links to article

kingdom) conducted by Jane Goodall (born 1934). 1977: DNA sequencing by chain termination developed by Frederick Sanger (1918–2013). Sanger won the Nobel Prize

NS5B inhibitor (3,798 words) [view diff] exact match in snippet view article find links to article

inhibitors, which contain a 3'-hydroxyl group, is the reason for the chain termination. Because of this mechanism, nucleoside inhibitors are sometimes called

Metabarcoding (11,273 words) [view diff] no match in snippet view article find links to article

techniques should be chosen for DNA sequencing. The classic Sanger chain‐termination method relies on the selective incorporation of chain‐elongating inhibitors