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searching for Microinjection 83 found (133 total)

Wingless localisation element 3 (WLE3) (281 words) [view diff] exact match in snippet view article

is shown to be sufficient and necessary for apical RNA transport in microinjection assays and transgenic reporters. Indeed, deletion of WLE3 in an otherwise

Nanopipette (299 words) [view diff] exact match in snippet view article find links to article

have a wide range of usage areas like electrophysiological settings, microinjection needles etc. After glass nanopipettes are fabricated and coated with

William French Anderson (4,093 words) [view diff] exact match in snippet view article find links to article

first approach for developing a human gene therapy procedure, standard microinjection techniques were modified to permit the injection of DNA plasmids into

Alan Hall (1,981 words) [view diff] exact match in snippet view article find links to article

proteins, at the intracellular face of the plasma membrane with Val14rhoA microinjection showed the association of focal adhesions with the end of the new stress

Intracytoplasmic sperm injection (3,636 words) [view diff] exact match in snippet view article find links to article

easily evaluated on the basis of sperm motility in most cases. The microinjection procedure for ROSI also differs slightly from that of ICSI, since additional

Phalloidin (1,696 words) [view diff] exact match in snippet view article find links to article

phalloidin are used. Consequently, immunofluorescence microscopy along with microinjection of phalloidin can be used to evaluate the direct and indirect functions

Transcription activator-like effector nuclease (3,148 words) [view diff] exact match in snippet view article find links to article

approach has also been used to generate knockin rats by TALEN mRNA microinjection in one-cell embryos. TALEN has also been utilized experimentally to

Median preoptic nucleus (2,518 words) [view diff] exact match in snippet view article find links to article

thermogenesis, as well as on wake-active areas of the brain to induce sleep. Microinjection of ethanol, triazolam, and propofol into this area induces sleep in

Nucleus raphe pallidus (304 words) [view diff] exact match in snippet view article find links to article

stress. Microinjections of a GABA-a antagonist into the nucleus raphe pallidus, induces an increased heart rate. Conversely, microinjections of muscimol

Nucleus raphe magnus (537 words) [view diff] exact match in snippet view article find links to article

Issue 6, December 2007, Pages 3121-33. Hellman, Kevin et al., Opioid microinjection into raphe magnus modulates cardiorespiratory function in mice and rats

Rostral ventromedial medulla (2,207 words) [view diff] exact match in snippet view article find links to article

majority of μ-opioid-expressing neurons also expressed CCK2 receptors. Microinjection in the RVM with either a CCK-saporin or a dermorphin-saporin conjugate

Xenbase (1,600 words) [view diff] exact match in snippet view article find links to article

among these is the robustness of early embryos and their amenability to microinjection and microsurgery. This makes them a particularly attractive system for

Mouse brain (625 words) [view diff] exact match in snippet view article find links to article

Ruddle, F. H. (1980-12-01). "Genetic transformation of mouse embryos by microinjection of purified DNA". Proceedings of the National Academy of Sciences. 77

U-69,593 (562 words) [view diff] exact match in snippet view article find links to article

PMID 7568631. S2CID 21326960. Wall PM, Messier C (February 2000). "U-69,593 microinjection in the infralimbic cortex reduces anxiety and enhances spontaneous alternation

Bacterial artificial chromosome (936 words) [view diff] exact match in snippet view article find links to article

electroporation/transformation, transfection with a suitable virus or microinjection. BACs can also be utilized to detect genes or large sequences of interest

Palatability (1,194 words) [view diff] exact match in snippet view article find links to article

accumbens shell mediates eating and hedonic 'liking' for food: Map based on microinjection Fos plumes". Brain Research. 863 (1–2): 71–86. doi:10.1016/s0006-8993(00)02102-8

Richard Harvey (scientist) (652 words) [view diff] exact match in snippet view article

ISSN 0305-1048. PMC 327051. PMID 6296794. Harvey, R.P.; Melton, D.A. (1988). "Microinjection of synthetic Xhox-1A homeobox mRNA disrupts somite formation in developing

Stria terminalis (1,443 words) [view diff] exact match in snippet view article find links to article

microinjection of oxytocin receptor antagonist had lower social recognition scores than rats that received a vehicle injection, and microinjections of

Maturation promoting factor (873 words) [view diff] exact match in snippet view article find links to article

that frog oocytes arrested in G2 could be induced to enter M phase by microinjection of cytoplasm from oocytes that had been hormonally stimulated with progesterone

Keith Burridge (1,447 words) [view diff] exact match in snippet view article find links to article

doi:10.1038/nrm2568. Burridge K, Feramisco JR; Feramisco (March 1980). "Microinjection and localization of a 130K protein in living fibroblasts: a relationship

Ralph L. Brinster (4,059 words) [view diff] exact match in snippet view article find links to article

RNA and DNA, and was at the forefront of the field in applying these microinjection methods to generate transgenic mice. Direct injection of fertilized

Ann Kiessling (1,570 words) [view diff] exact match in snippet view article find links to article

PMID 2464685. O'Keefe SJ, Wolfes H, Kiessling AA, Cooper GM (September 1989). "Microinjection of antisense c-mos oligonucleotides prevents meiosis II in the maturing

Neuropeptide S (1,144 words) [view diff] exact match in snippet view article find links to article

PMID 18793688. S2CID 207356961. Mochizuki T, Kim J, Sasaki K (May 2010). "Microinjection of neuropeptide S into the rat ventral tegmental area induces hyperactivity

CDC25C (1,289 words) [view diff] exact match in snippet view article find links to article

Capony JP, et al. (1994). "Activation of p34cdc2 protein kinase by microinjection of human cdc25C into mammalian cells. Requirement for prior phosphorylation

Mario Capecchi (1,969 words) [view diff] exact match in snippet view article find links to article

Capecchi, M. R. (1980). "High efficiency transformation by direct microinjection of DNA into cultured mammalian cells". Cell. 22 (2 Pt 2): 479–488. doi:10

Cytolethal distending toxin (1,897 words) [view diff] exact match in snippet view article find links to article

canonical ones. CdtB is considered the active subunit of the CDT holotoxin. Microinjection of CdtB into susceptible cells without CdtA or CdtC results in the G2/M

Optical transfection (2,127 words) [view diff] exact match in snippet view article find links to article

Hanpeng; Yang, Hao; Yao, Baoli (2008). "Femtosecond laser-assisted microinjection into living neurons". Journal of Neuroscience Methods. 174 (2). Elsevier

Charles Daniel Lane (1,864 words) [view diff] exact match in snippet view article find links to article

Christ Church, Oxford. His University of Oxford doctoral thesis was "The Microinjection of RNA into Eggs and Oocytes of Xenopus Laevis". A hard copy of the

Phosphatidylinositol 3,5-bisphosphate (2,639 words) [view diff] exact match in snippet view article find links to article

depletion is reversible and could be selectively rescued by cytosolic microinjection of PtdIns(3,5)P2, overexpression of PIKfyve or wash-out of the PIKfyve

Centre for Genomic Regulation (916 words) [view diff] exact match in snippet view article find links to article

fluorescence microscopy, fluorescence lifetime imaging microscopy, microinjection, microdissection, image processing and analysis, and other areas. The

PCM1 (1,718 words) [view diff] exact match in snippet view article find links to article

using siRNA, overexpression of PCM1 deletion mutants and PCM1 antibody microinjection. As a result of this depletion, the radial organization of the microtubules

Rho family of GTPases (3,212 words) [view diff] exact match in snippet view article find links to article

Paterson HF, Self AJ, Garrett MD, Just I, Aktories K, Hall A (1990). "Microinjection of recombinant p21 rho induces rapid changes in cell morphology". J

Nystatin (2,621 words) [view diff] exact match in snippet view article find links to article

Perforated Patch Recording", Methods in Neurosciences, Electrophysiology and Microinjection, vol. 4, Academic Press, pp. 364–373, doi:10.1016/B978-0-12-185257-3

Fox Chase Cancer Center (2,104 words) [view diff] exact match in snippet view article find links to article

Mintz, B. (November 1979). "Prevention of genetic anemias in mice by microinjection of normal hematopoietic stem cells into the fetal placenta". Proceedings

Douglas A. Melton (1,197 words) [view diff] exact match in snippet view article find links to article

PMID 7039494. Wikidata Q28277421. R P Harvey; D A Melton (3 June 1988). "Microinjection of synthetic Xhox-1A homeobox mRNA disrupts somite formation in developing

Glutamate (neurotransmitter) (2,177 words) [view diff] exact match in snippet view article

expression. Glutamic acid has been implicated in epileptic seizures. Microinjection of glutamic acid into neurons produces spontaneous depolarisations around

Julian Hibberd (1,006 words) [view diff] exact match in snippet view article find links to article

C.; Van Bel, A. J. (1999). "A galinstan expansion femtosyringe for microinjection of eukaryotic organelles and prokaryotes". Nature Biotechnology. 17

PRKAR1B (1,198 words) [view diff] exact match in snippet view article find links to article

cAMP-dependent protein kinases: expression, holoenzyme formation and microinjection into living cells". Exp. Cell Res. 214 (2): 595–605. doi:10.1006/excr

Parthenogenesis (6,355 words) [view diff] exact match in snippet view article find links to article

activation that mimics sperm entry, such as calcium ionophore treatment, microinjection of calcium ions, or electrical stimulation. Treatment with cycloheximide

Fluorescent tag (2,782 words) [view diff] exact match in snippet view article find links to article

that is chemically coupled with a fluorescent tag into living cells by microinjection. This technique was used to show how the oskar mRNA in the Drosophila

GLE1L (661 words) [view diff] exact match in snippet view article find links to article

sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of

Oklahoma Medical Research Foundation (1,588 words) [view diff] exact match in snippet view article find links to article

hybridization histochemistry; Mouse Genome Manipulation Facility, providing microinjection services of DNA into zygotes for the generation of transgenic mice,

Peter K. Hepler (5,059 words) [view diff] exact match in snippet view article find links to article

observed calcium ion transients during mitosis and showed that the microinjection of calcium ions into the mitotic spindle does regulate the depolymerization

Helen Sang (973 words) [view diff] exact match in snippet view article find links to article

PMC 1299092. PMID 15192698. Sang, Helen (1994). "Transgenic Birds by DNA Microinjection". EMBO Reports. 12 (1): 60–63. doi:10.1038/nbt0194-60. PMID 7764327

Vinculin (2,985 words) [view diff] exact match in snippet view article find links to article

PMID 574428. S2CID 33153559. Burridge K, Feramisco JR (March 1980). "Microinjection and localization of a 130K protein in living fibroblasts: a relationship

Maria Byrne (biologist) (1,422 words) [view diff] exact match in snippet view article

Allison; Byrne, Maria; Wray, Gregory A. (17 September 2019). "Embryo microinjection of the lecithotrophic sea urchin Heliocidaris erythrogramma". Journal

Carl Wood (1,068 words) [view diff] exact match in snippet view article find links to article

first IVF baby using sperm retrieval surgery 1986 and world's first Microinjection Intra Fallopian Transfer (MIFT) IVF baby 1992. The most important development

Capitella teleta (4,604 words) [view diff] exact match in snippet view article find links to article

developed as a way to visualize specific cell types in fixed specimens. A microinjection protocol for uncleaved embryos and early cleavage stages was developed

Kinetochore (7,831 words) [view diff] exact match in snippet view article find links to article

impaired function of Ndc80 (using RNAi, gene knockout, or antibody microinjection) have abnormally long spindles, lack of tension between sister kinetochores

Golgi matrix (1,425 words) [view diff] exact match in snippet view article find links to article

Microinjection of antibodies to GRASP65 prevents normal Golgi stack formation.

Organoid (9,001 words) [view diff] exact match in snippet view article find links to article

infection via a more natural route starting from the intestinal lumen, microinjection of the pathogen is required. In addition, the polarity of intestinal

Restriction enzyme (5,853 words) [view diff] exact match in snippet view article find links to article

Miller JC, Choi VM, et al. (July 2009). "Knockout rats via embryo microinjection of zinc-finger nucleases". Science. 325 (5939): 433. Bibcode:2009Sci

Drosophila (6,594 words) [view diff] exact match in snippet view article find links to article

Bangalore, India. Archived from the original on 2013-01-21. – In India microinjection service for the generation of transgenic lines, Screening Platforms

Zinc finger (4,879 words) [view diff] exact match in snippet view article find links to article

Urnov FD, Jacob HJ, Buelow R (July 2009). "Knockout rats via embryo microinjection of zinc-finger nucleases". Science. 325 (5939): 433. Bibcode:2009Sci

Transcription activator-like effector (2,796 words) [view diff] exact match in snippet view article find links to article

BJ, Remy S, et al. (August 2011). "Knockout rats generated by embryo microinjection of TALENs". Nature Biotechnology. 29 (8): 695–6. doi:10.1038/nbt.1940

IFRD1 (1,603 words) [view diff] exact match in snippet view article find links to article

function in C2C12 myoblasts, by antisense IFRD1 cDNA transfection or microinjection of anti-IFRD1 antibodies, prevents their morphological and biochemical

Ann E. Kelley (1,269 words) [view diff] exact match in snippet view article find links to article

"Enhanced intake of high-fat food following striatal mu-opioid stimulation: Microinjection mapping and Fos expression". Neuroscience. 99 (2): 267–277. doi:10

Aurora kinase B (4,535 words) [view diff] exact match in snippet view article find links to article

kinetochores. Inhibition of Aurora B function by RNA interference or microinjection of blocking antibodies impairs the alignment of chromosomes at the equator

Xenopus (5,807 words) [view diff] exact match in snippet view article find links to article

manner. They remain stable in cells and do not induce immune responses. Microinjection of MOs in early Xenopus embryos can suppress gene expression in a targeted

Slow-wave sleep (6,253 words) [view diff] exact match in snippet view article find links to article

have widespread projections throughout the brain. ... Intraventricular microinjection of MCH increases both slow wave sleep (SWS) and REM sleep; however,

Sperm-mediated gene transfer (1,053 words) [view diff] exact match in snippet view article find links to article

cost-effective method of gene therapy, especially in contrast with pronuclear microinjection, another transgenic technique. Nevertheless, despite some successes

PTPN1 (2,731 words) [view diff] exact match in snippet view article find links to article

MF, Tonks NK, Diltz CD, Weiel JE, Fischer EH, Krebs EG (July 1990). "Microinjection of a protein-tyrosine-phosphatase inhibits insulin action in Xenopus

Zinc-finger nuclease (4,665 words) [view diff] exact match in snippet view article find links to article

PD, Urnov FD, Jacob HJ, Buelow R (2009). "Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases". Science. 325 (5939): 433. Bibcode:2009Sci

Transposons as a genetic tool (2,133 words) [view diff] exact match in snippet view article find links to article

gene of interest (with a broken transposase) you can remobilise by microinjection of the embryo with coding for transposase or transposase itself. The

Clostridium novyi (2,002 words) [view diff] exact match in snippet view article find links to article

(July 1992). "Morphological changes of cultured endothelial cells after microinjection of toxins that act on the cytoskeleton". Infection and Immunity. 60

CREB-binding protein (5,272 words) [view diff] exact match in snippet view article find links to article

affinity. A knockout study that was published in 2000, which used a microinjection of an antibody against CBP/p300 significantly diminished the number

Wallace B. Mendelson (1,441 words) [view diff] exact match in snippet view article find links to article

Mendelson, W.B. (February 16, 2001). "The sleep-inducing effect of ethanol microinjection into the medial preoptic area is blocked by flumazenil". Brain Research

U7 small nuclear RNA (3,630 words) [view diff] exact match in snippet view article find links to article

(1995). "Assembly, nuclear import and function of U7 snRNPs studied by microinjection of synthetic U7 RNA into Xenopus oocytes". Nucleic Acids Res. 23 (16):

Recombinant AAV mediated genome engineering (2,673 words) [view diff] exact match in snippet view article find links to article

Cost GJ, Freyvert Y, et al. (July 2009). "Knockout rats via embryo microinjection of zinc-finger nucleases". Science. 325 (5939): 433. Bibcode:2009Sci

Wolbachia (8,675 words) [view diff] exact match in snippet view article find links to article

infection in Aedes albopictus (Asian tiger mosquito) via embryonic microinjection". Insect Biochemistry and Molecular Biology. 35 (8): 903–910. Bibcode:2005IBMB

Kaang Bong-kiun (2,028 words) [view diff] exact match in snippet view article find links to article

mechanisms of learning and memory. In his Ph.D. course, he developed the microinjection-based gene transfer method for Aplysia neurons. This technology opened

George Scangos (686 words) [view diff] exact match in snippet view article find links to article

Ruddle, F H (December 1980). "Genetic transformation of mouse embryos by microinjection of purified DNA". Proceedings of the National Academy of Sciences. 77

Aedes albopictus (8,931 words) [view diff] exact match in snippet view article find links to article

infection in Aedes albopictus (Asian tiger mosquito) via embryonic microinjection". Insect Biochemistry and Molecular Biology. 35 (8): 903–910. doi:10

Microdialysis (3,787 words) [view diff] exact match in snippet view article find links to article

pressure artifacts, which can occur when using other techniques, such as microinjection or push-pull perfusion. The semipermeable membrane prevents cells, cellular

Janet E. Mertz (1,132 words) [view diff] exact match in snippet view article find links to article

Mertz & John B. Gurdon (1977) Purified DNAs are transcribed after microinjection into Xenopus oocytes. Proc Natl Acad Sci USA 74:1502-1506. Edward M

Nicotinic acid adenine dinucleotide phosphate (6,140 words) [view diff] exact match in snippet view article find links to article

membrane-impermeant analog of NAADP that can be introduced into cells by microinjection or a patch pipette. Flash photolysis with a UV light source rapidly

Simon Fishel (2,041 words) [view diff] exact match in snippet view article find links to article

Instruments to help him develop tools for the earliest beginnings of sperm microinjection. However, British regulators would not grant Fishel a licence for a

PURA (4,047 words) [view diff] exact match in snippet view article find links to article

(July 1999). "Cell cycle arrest and morphological alterations following microinjection of NIH3T3 cells with Pur alpha". Oncogene. 18 (29): 4254–61. doi:10

Zinc finger chimera (4,966 words) [view diff] exact match in snippet view article find links to article

Cost GJ, Freyvert Y, et al. (July 2009). "Knockout rats via embryo microinjection of zinc-finger nucleases". Science. 325 (5939): 433. Bibcode:2009Sci

Acanthocheilonema (4,361 words) [view diff] exact match in snippet view article find links to article

Theuring, F.; Kalinna, B. H. (2003). "Trans-species transfer of Wolbachia: Microinjection of Wolbachia from Litomosoides sigmodontis into Acanthocheilonema viteae"

Glossary of cellular and molecular biology (M–Z) (24,991 words) [view diff] exact match in snippet view article

accumulated nucleic acids away from the lance and thus into the cell. Compare microinjection. nascent In the process of being synthesized; incomplete; not yet fully

Peter Kalivas (2,105 words) [view diff] exact match in snippet view article find links to article

A.J. (1983). "Behavioral and neurochemical effects of neurotensin microinjection into the ventral tegmental area of the rat". Neuroscience. 8 (3): 495–505

Communist mummies (2,283 words) [view diff] exact match in snippet view article find links to article

autopsy, so he wears a double-layered rubber suit and scientists use microinjection techniques to deliver embalming fluids. According to anthropologist