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Longer titles found: Restriction enzyme mediated integration (view), List of restriction enzyme cutting sites (view), List of restriction enzyme cutting sites: Bsa–Bso (view), List of restriction enzyme cutting sites: T–Z (view), List of restriction enzyme cutting sites: Bd–Bp (view), List of restriction enzyme cutting sites: Ba–Bc (view), List of restriction enzyme cutting sites: G–K (view), List of restriction enzyme cutting sites: C–D (view), List of restriction enzyme cutting sites: L–N (view), List of restriction enzyme cutting sites: Bsp–Bss (view), List of restriction enzyme cutting sites: E–F (view), List of restriction enzyme cutting sites: A (view), List of restriction enzyme cutting sites: O–R (view), List of restriction enzyme cutting sites: Bst–Bv (view), List of restriction enzyme cutting sites: S (view)

searching for Restriction enzyme 81 found (263 total)

alternate case: restriction enzyme

Palindromic sequence (825 words) [view diff] exact match in snippet view article find links to article

enzymes) recognize specific palindromic sequences and cut them. The restriction enzyme EcoR1 recognizes the following palindromic sequence: 5'- G A A T T
Representational oligonucleotide microarray analysis (416 words) [view diff] exact match in snippet view article find links to article
has the advantage of reducing the complexity of a genome with a restriction enzyme which highly increases the efficiency of genomic fragment hybridization
Ligation-independent cloning (195 words) [view diff] exact match in snippet view article find links to article
The technique was developed in the early 1990s as an alternative to restriction enzyme/ligase cloning. This allows genes to be cloned without the requirement
Optical mapping (2,102 words) [view diff] exact match in snippet view article find links to article
molecules of DNA, called "optical maps". By mapping the location of restriction enzyme sites along the unknown DNA of an organism, the spectrum of resulting
Gateway Technology (1,359 words) [view diff] exact match in snippet view article find links to article
more time for initial set-up, and is more expensive than traditional restriction enzyme and ligase-based cloning methods, but it saves time and offers simpler
Cleaved amplified polymorphic sequence (158 words) [view diff] exact match in snippet view article find links to article
the altered restriction site, and the products digested with the restriction enzyme. When fractionated by agarose or polyacrylamide gel electrophoresis
Bacillus amyloliquefaciens (1,214 words) [view diff] exact match in snippet view article find links to article
of bacterium in the genus Bacillus that is the source of the BamHI restriction enzyme. It also synthesizes a natural antibiotic protein barnase, a widely
Combined bisulfite restriction analysis (1,300 words) [view diff] exact match in snippet view article find links to article
to the methylation dependent retention or loss of CpG-containing restriction enzyme sites, such as those for TaqI (TCGA) and BstUI (CGCG), depending on
Gibson assembly (689 words) [view diff] exact match in snippet view article find links to article
DNA assembly method has many advantages compared to conventional restriction enzyme/ligation cloning of recombinant DNA. For example, No restriction digest
Brevinema andersonii (874 words) [view diff] exact match in snippet view article find links to article
laboratory mice and Syrian hamsters.B. andersonii is readily identified by restriction enzyme analysis, and SDS-PAGE, or fatty acid composition data. Another identifier
CAG promoter (445 words) [view diff] exact match in snippet view article find links to article
proximal region of the second exon was disrupted by digesting with NcoI restriction enzyme and replacing the site with a HindIII linker. Although the whole construct
Restriction endonuclease BsobI/AvaI (102 words) [view diff] exact match in snippet view article find links to article
van der Woerd MJ, Pelletier JJ, Xu S, Friedman AM (February 2001). "Restriction enzyme BsoBI-DNA complex: a tunnel for recognition of degenerate DNA sequences
Disciotis (215 words) [view diff] exact match in snippet view article find links to article
Morchella, Verpa, and Disciotis (Pezizales: Morchellaceae) based on restriction enzyme analysis of the 28S ribosomal RNA gene. Experimental Mycology 19 (3):
Morchellaceae (393 words) [view diff] exact match in snippet view article find links to article
Morchella, Verpa, and Disciotis (Pezizales: Morchellaceae) based on restriction enzyme analysis of the 28S ribosomal RNA gene". Experimental Mycology. 19
List of online databases (489 words) [view diff] case mismatch in snippet view article find links to article
PsycINFO PubChem PubMed Central Questia – defunct Rate Your Music REBASE Restriction Enzyme Database RedLightGreen Reptile Database Roller Coaster DataBase Roud
Coprinopsis cinerea (1,455 words) [view diff] exact match in snippet view article find links to article
be used, likely with low transformation efficiency. Earlier, REMI (restriction enzyme-mediated integration) could be used to insert exogenous DNA into the
B3 domain (734 words) [view diff] exact match in snippet view article find links to article
G, Huber R, Siksnys V (2005). "Structure of the metal-independent restriction enzyme BfiI reveals fusion of a specific DNA-binding domain with a nonspecific
Michael McClelland (academic) (867 words) [view diff] case mismatch in snippet view article
and Restriction Enzyme Recognition Sequences. IRL Press. 1989. TRNA, Cloned Human DNA and E. Coli Sequences, Histone Genes and Restriction Enzyme Recognition
EMBOSS (324 words) [view diff] exact match in snippet view article find links to article
Nucleic repeats Nucleic acid repeat detection Nucleic restriction Restriction enzyme sites in nucleotide sequences Nucleic RNA folding RNA folding methods
Nicking enzyme (437 words) [view diff] exact match in snippet view article find links to article
Shank DD (Jan 1992). "Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system". Proc. Natl. Acad. Sci. U.S.A. 89 (1): 392–6
Haplogroup R2 (490 words) [view diff] exact match in snippet view article find links to article
(bp) reference sequence 323 Polymorphism position from 5' end 107 Restriction enzyme variant HphI RefSNP ID - Y-position 19294055 Primer forward 5'-3'
BLESS (1,978 words) [view diff] exact match in snippet view article find links to article
barcode sequence that labels the site of ligation as well as an XhoI restriction enzyme recognition site adjacent to the barcode. The hairpin loop of the
Fasciola gigantica (1,224 words) [view diff] exact match in snippet view article find links to article
differentiation of Fasciola hepatica and Fasciola gigantica using a simple PCR-restriction enzyme method". Experimental Parasitology. 124 (2): 209–213. doi:10.1016/j
Nannizzia fulva (89 words) [view diff] exact match in snippet view article find links to article
"Phylogeny of Nannizzia incurvata, N. gypsea, N. fulva and N. otae by restriction enzyme analysis of mitochondrial DNA". Mycopathologia. 112 (3): 173–177.
Epidermophyton floccosum (1,511 words) [view diff] exact match in snippet view article find links to article
distinguishes between 12 dermatophyte species based on their individual restriction enzyme profiles, including one for E. floccosum. A real time PCR protocol
Nannizzia (234 words) [view diff] exact match in snippet view article find links to article
"Phylogeny of Nannizzia incurvata, N. gypsea, N. fulva and N. otae by restriction enzyme analysis of mitochondrial DNA". Mycopathologia. 112 (3): 173–177.
Protein engineering (7,486 words) [view diff] exact match in snippet view article find links to article
N-terminus. These hybrid gene fragments are separated using either restriction enzyme digestion or PCR with terminus primers via agarose gel electrophoresis
Morchella elata (1,566 words) [view diff] exact match in snippet view article find links to article
Morchella, Verpa, andDisciotis (Pezizales: Morchellaceae) based on restriction enzyme analysis of the 28S ribosomal RNA gene". Experimental Mycology. 19
TOPO cloning (568 words) [view diff] exact match in snippet view article find links to article
adenine-overhangs. The target vector is linearized and cut with a blunt-end restriction enzyme. This vector is then tailed with dideoxythymidine triphosphate (ddTTP)
Nannizzia incurvata (164 words) [view diff] exact match in snippet view article find links to article
"Phylogeny of Nannizzia incurvata, N. gypsea, N. fulva and N. otae by restriction enzyme analysis of mitochondrial DNA". Mycopathologia. 112 (3): 173–177.
Population genomics (2,174 words) [view diff] exact match in snippet view article find links to article
genetic variability at multiple loci included gel electrophoresis and restriction enzyme mapping. Previously genomics was restricted to only the study of a
Overlap extension polymerase chain reaction (1,052 words) [view diff] exact match in snippet view article find links to article
as Gibson assembly, which overcome the limitations of traditional restriction enzyme digestion and ligation cloning methods. Assembly of custom DNA sequences
Tapesia yallundae (665 words) [view diff] exact match in snippet view article find links to article
compact and mounded. Polymerase chain reaction (PCR) combined with restriction enzyme digestion of an amplified ribosomal DNA fragment, are now used to
V. Nagaraja (635 words) [view diff] exact match in snippet view article find links to article
DNA transaction enzymes, design and development of high fidelity restriction enzyme, new inhibitors for the generation of lead molecules as a step for
Computational epigenetics (1,098 words) [view diff] case mismatch in snippet view article find links to article
Methylation Database Pubmeth.Org "Official REBASE Homepage | the Restriction Enzyme Database | NEB". "DeepBlue Epigenomic Data Server". "MeInfoText: associated
HLA-DQA2 (1,251 words) [view diff] exact match in snippet view article find links to article
"Sequences related to HLA-DR alpha chain on human chromosome 6: restriction enzyme polymorphism detected with DC alpha chain probes". Proceedings of
Sulfolobus acidocaldarius (956 words) [view diff] exact match in snippet view article find links to article
rescues Sulfolobales from DNA damaging threats. The thermostable restriction enzyme SuaI is obtained from this organism. Brock, TD; Brock, KM; Belly,
Natural competence (1,906 words) [view diff] exact match in snippet view article find links to article
3-Sex pili 4-Plasmid of foreign DNA from a dead cell 5-Bacterial cell restriction enzyme 6-Unwound foreign plasmid 7-DNA ligase I: A plasmid of foreign DNA
Site-specific DNA-methyltransferase (cytosine-N4-specific) (249 words) [view diff] case mismatch in snippet view article
PMID 6267988. Roberts RJ. "A complete listing of all of these enzymes". The Restriction Enzyme Database. Site-specific+DNA-methyltransferase+(cytosine-N4-specific)
Addgene (832 words) [view diff] exact match in snippet view article find links to article
scientists design plasmid cloning experiments, including tutorials on restriction enzyme digestion and PCR-based cloning. Molecular Cloning Protocols—Specific
HLA-DQ1 (1,482 words) [view diff] exact match in snippet view article find links to article
"Sequences related to HLA-DR alpha chain on human chromosome 6: restriction enzyme polymorphism detected with DC alpha chain probes". Proc. Natl. Acad
Biotechnology in pharmaceutical manufacturing (1,536 words) [view diff] exact match in snippet view article find links to article
found in pituitary glands to complementary DNA. HaeIII, a type of restriction enzyme which acts at restriction sites "in the 3' noncoding region" and at
Factor XII (3,097 words) [view diff] exact match in snippet view article find links to article
1987). "Factor XII gene alteration in Hageman trait detected by TaqI restriction enzyme". Blood. 69 (5): 1421–1424. doi:10.1182/blood.V69.5.1421.1421. PMID 2882793
Ground squirrel hepatitis virus (1,735 words) [view diff] exact match in snippet view article find links to article
are similar to HBV and WHV, but the viruses differ in their EcoRI restriction enzyme recognition sites. Ground squirrel hepatitis virus is a spherical
Reverse genetics (2,635 words) [view diff] exact match in snippet view article find links to article
RNA polymerase I (Pol I) promoter and terminator sequence through restriction enzyme digestion. The cDNA and pol I sequence is then, in turn, surrounded
Recombinant DNA (3,852 words) [view diff] exact match in snippet view article find links to article
DNA segments can be combined by using a variety of methods, such as restriction enzyme/ligase cloning or Gibson assembly.[citation needed] In standard cloning
Aedes tormentor (240 words) [view diff] exact match in snippet view article find links to article
distinguish between the two species. Researchers have developed a restriction enzyme assay based on DNA sequence data to differentiate between A. atlanticus
Salvelinus neiva (187 words) [view diff] case mismatch in snippet view article find links to article
8 December 2023. Radchenko, O. A. (1 January 2003). "Comparative Restriction Enzyme Analysis of Mitochondrial DNA in Several Populations of Lake Resident
Entamoeba histolytica (3,032 words) [view diff] exact match in snippet view article find links to article
Rao DN, Bhattacharya S (December 2009). "Characterization of the restriction enzyme-like endonuclease encoded by the Entamoeba histolytica non-long terminal
Maryland v. King (1,882 words) [view diff] exact match in snippet view article find links to article
The DNA from the cheek cells in the swab is replicated, given a restriction enzyme digest and electrophoresed. Electrophoresis separates DNA segments
COLD-PCR (1,607 words) [view diff] exact match in snippet view article find links to article
(or absence thereof) of DNA for a specific mutation by a selected restriction enzyme that will not cleave the wildtype DNA. In a study using a mixture
Genetically modified virus (3,843 words) [view diff] exact match in snippet view article find links to article
into a viral genome was published, when Paul Berg used the EcoRI restriction enzyme and DNA ligases to create the first ever recombinant DNA molecules
Mobile genetic elements (3,789 words) [view diff] exact match in snippet view article find links to article
vector plasmid. This vector contains the origin of replication sequence, the restriction enzyme cut site, lacZ gene, and an ampicillin resistance gene.
Rolling circle replication (2,920 words) [view diff] exact match in snippet view article find links to article
amplification or C2CA, where the RCA products are digested with a restriction enzyme and ligated into new circular templates using a restriction oligo
Stanley Falkow (4,189 words) [view diff] exact match in snippet view article find links to article
Waikiki beach meeting in 1972 between Herb Boyer (who provided the restriction enzyme EcoRI) and Stanley Cohen (who provided the plasmid) that led to a
Chromatin (6,008 words) [view diff] case mismatch in snippet view article find links to article
Randall H. (ed.), "Assaying Chromatin Structure and Remodeling by Restriction Enzyme Accessibility", Chromatin Remodeling, Methods in Molecular Biology
Joanna Wilson (921 words) [view diff] exact match in snippet view article find links to article
Y Wilson, Christopher M Somers. 2015. Impacts of degraded DNA on restriction enzyme associated DNA sequencing (RADS eq). Molecular Ecology Resources,
Pelham Memorial High School (3,247 words) [view diff] exact match in snippet view article find links to article
techniques, such as DNA amplification via polymerase chain reaction, restriction enzyme analysis, and bacterial culture, identification, and transformation
Morchella (6,434 words) [view diff] exact match in snippet view article find links to article
Morchella, Verpa, andDisciotis (Pezizales: Morchellaceae) based on restriction enzyme analysis of the 28S ribosomal RNA gene". Experimental Mycology. 19
PTC tasting (1,442 words) [view diff] exact match in snippet view article find links to article
TAS2R38. The amplified fragment (amplicon) is incubated with the restriction enzyme HaeIII, comprising the SNP in their recognition sequence GGCC. HaeIII
Thomas J. Kelly (scientist) (1,237 words) [view diff] exact match in snippet view article
on March 5, 2013 Kelly, Thomas J.; Smith, Hamilton O. (1970). "A restriction enzyme from Hemophilus influenzae: II. Base sequence of the recognition site"
Mayan genetics (1,497 words) [view diff] exact match in snippet view article find links to article
and D.. Haplogroup A, (np) 16111 mutation of C to T and a Hae III Restriction enzyme site at np 633. mtDNA HincII morph-6 (site loss at np 13 259) and
DNA adenine methylase (1,844 words) [view diff] case mismatch in snippet view article find links to article
50.070181.001441. PMID 6267988. Roberts RJ, Macelis D (eds.). "The Restriction Enzyme Database". REBASE. Retrieved 22 Feb 2020. Aughey GN, Southall TD (January
Rhesus macaque (8,597 words) [view diff] exact match in snippet view article find links to article
rheusus monkeys (Macaca mulatta) as revealed by mitochondrial DNA restriction enzyme analysis". International Journal of Primatology. 14 (4): 587. doi:10
Arthur Riggs (geneticist) (2,448 words) [view diff] exact match in snippet view article
hypothesized that X chromosome inactivation might act in ways analogous to restriction enzyme complexes such as E. coli. He eventually published a theoretical paper
APOBEC3A (1,699 words) [view diff] exact match in snippet view article find links to article
"Methylcytosine and normal cytosine deamination by the foreign DNA restriction enzyme APOBEC3A". The Journal of Biological Chemistry. 287 (41): 34801–8
Raccoonpox virus (1,942 words) [view diff] exact match in snippet view article find links to article
of the genus. Differences between terminal region patterns of DNA restriction enzyme digests of different RCN isolates suggest that varied passaging has
Ligation (molecular biology) (4,729 words) [view diff] exact match in snippet view article
The information on the minimum basepair required is available from restriction enzyme suppliers such as in the catalog of New England Biolabs. Incomplete
In vitro compartmentalization (2,195 words) [view diff] exact match in snippet view article find links to article
expressed methyltransferase, would be methylated and, thus, resistant to restriction enzyme digestion. By selecting for DNA sequences that survive the endonuclease
Human variability (5,443 words) [view diff] exact match in snippet view article find links to article
human population studies based on mitochondrial DNA were performed by restriction enzyme analyses (RFLPs) and revealed differences between the four ethnic
Masai ostrich (2,515 words) [view diff] no match in snippet view article find links to article
blue-necked forms (S. c. australis and S. c. molybdyphanes). Comparative restriction-enzyme analysis of mitochondrial DNA (mtDNA) found the Masai ostrich, though
Histophilus somni (3,433 words) [view diff] exact match in snippet view article find links to article
differentiation between ovine and bovine strains of H. somni, including restriction enzyme analysis. Microscopy can be used for identification when bacteria
Artificial gene synthesis (7,073 words) [view diff] exact match in snippet view article find links to article
negatively affect protein folding or expression. By using the BsaI restriction enzyme that produces a 4 base pair overhang, up to 240 unique, non-palindromic
Whole genome bisulfite sequencing (2,517 words) [view diff] exact match in snippet view article find links to article
methylation levels at single-CpG resolution from methylation enrichment and restriction enzyme sequencing methods". Genome Research. 23 (9): 1541–1553. doi:10.1101/gr
Zinc finger nuclease treatment of HIV (3,400 words) [view diff] exact match in snippet view article find links to article
restriction modification (R-M) systems. These bacteria secrete a restriction enzyme (REase) that recognizes and repetitively cleaves around palindromic
Zeda Rosenberg (2,427 words) [view diff] exact match in snippet view article find links to article
355-359 (1980). Sahagan, B.G., Rosenberg, Z.F., and Haseltine, W.A. Restriction enzyme analysis of the feline sarcoma virus provirus in murine fibroblasts
Magnetic tweezers (5,021 words) [view diff] exact match in snippet view article find links to article
more about DNA-binding proteins, receptor-ligand interactions, and restriction enzyme cleavage. A more recent application of magnetic tweezers is seen in
Glossary of cellular and molecular biology (0–L) (29,839 words) [view diff] exact match in snippet view article
synthetic DNA duplex containing the recognition sequence for a particular restriction enzyme. In molecular cloning, linkers are often deliberately included in
Nucleoid (17,312 words) [view diff] exact match in snippet view article find links to article
between genomic loci. Subsequently, the genome is digested with a restriction enzyme. In the next step, a DNA ligation is carried out under diluted DNA
Actinobacillus equuli (4,981 words) [view diff] exact match in snippet view article find links to article
from the oral cavity of healthy horses and comparison of isolates by restriction enzyme digestion and Pulsed-Field Gel Electrophoresis". Veterinary Microbiology
Healthier Lives (5,627 words) [view diff] exact match in snippet view article find links to article
Basharat; Print, Cristin; Jones, Gregory T. (25 March 2024). "A validated restriction enzyme ddPCR cg05575921 (AHRR) assay to accurately assess smoking exposure"