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searching for Expression vector 47 found (76 total)

Immunoscreening (241 words) [view diff] exact match in snippet view article find links to article

strategy requires first that a gene library is implemented in an expression vector, and that antiserum to the protein is available. Radioactivity or

Monobody (1,490 words) [view diff] exact match in snippet view article find links to article

a cell of choice by simply transfecting the cell with a monobody expression vector. This is because of the characteristics of the underlying FN3 scaffold:

Pseudomonas aeruginosa hol holin family (348 words) [view diff] exact match in snippet view article find links to article

residues (aas)). Hol by itself, when expressed in a broad host-range expression vector under IPTG control exhibited strong lytic activity in both P. aeruginosa

Transmissible gastroenteritis virus (1,145 words) [view diff] exact match in snippet view article find links to article

The Transmissible Gastroenteritis Virus has been engineered as an expression vector. The vector was constructed by replacing the nonessential 3a and 3b

T7 expression system (798 words) [view diff] exact match in snippet view article find links to article

has generated over $55 million for the lab. An expression vector, most commonly the pET expression vector, is engineered to integrate two essential components:

Xylose metabolism (1,347 words) [view diff] exact match in snippet view article find links to article

"Overproduction of pentose phosphate pathway enzymes using a new CRE-loxP expression vector for repeated genomic integration in Saccharomyces cerevisiae". Yeast

Episome (512 words) [view diff] exact match in snippet view article find links to article

(March 2010). "pEPito: a significantly improved non-viral episomal expression vector for mammalian cells". BMC Biotechnology. 10 (1): 20. doi:10.1186/1472-6750-10-20

Japan Academy Prize (academics) (1,366 words) [view diff] case mismatch in snippet view article

of Paramyxovirus Pathogenicity and Generation of a Novel Class of Expression Vector" 2007 (97th) Senzô Hidemura - Agriculture and Society at the Last

RNA interference (15,445 words) [view diff] exact match in snippet view article find links to article

for gene suppression. Antisense RNA produced intracellularly by an expression vector may be developed and find utility as novel therapeutic agents. Two

IGHG4 (360 words) [view diff] exact match in snippet view article find links to article

Casadevall A, Green NS (Oct 2000). "Human and murine immunoglobulin expression vector cassettes". Molecular Immunology. 37 (14): 837–45. doi:10.1016/S0161-5890(00)00101-2

Viral vector vaccine (3,317 words) [view diff] exact match in snippet view article find links to article

(December 1982). "Vaccinia virus: a selectable eukaryotic cloning and expression vector". Proceedings of the National Academy of Sciences of the United States

Perturb-seq (2,588 words) [view diff] exact match in snippet view article find links to article

which are listed on the CRISPR/cas9 tools Wikipedia page. The sgRNA expression vector design will depend largely on the experiment performed but requires

Baculoviridae (2,591 words) [view diff] exact match in snippet view article find links to article

of H5N1 avian influenza in chickens was produced in a baculovirus expression vector). More recently, baculoviruses were found to transduce mammalian cells

Uroplakin-2 (651 words) [view diff] exact match in snippet view article find links to article

transitional cell carcinoma of bladder and construction of its eukaryotic expression vector". J. Huazhong Univ. Sci. Technol. Med. Sci. 25 (2): 188–90, 211. doi:10

IGHG1 (870 words) [view diff] exact match in snippet view article find links to article

Casadevall A, Green NS (2001). "Human and murine immunoglobulin expression vector cassettes". Mol. Immunol. 37 (14): 837–45. doi:10.1016/S0161-5890(00)00101-2

Virusoid (2,615 words) [view diff] exact match in snippet view article find links to article

overexpression and silencing of foreign genes. The unique example of a foreign expression vector is Bamboo mosaic virus satellite RNA (satBaMV), which possesses an

IGHG2 (779 words) [view diff] exact match in snippet view article find links to article

Casadevall A, Green NS (Oct 2000). "Human and murine immunoglobulin expression vector cassettes". Molecular Immunology. 37 (14): 837–45. doi:10.1016/S0161-5890(00)00101-2

DNA construct (1,699 words) [view diff] no match in snippet view article find links to article

genomic libraries, sequencing cloned DNA, and studying RNA and protein expression. Vector (molecular biology) Pinkert, Carl (2014). Transgenic animal technology:

Linda King (virologist) (296 words) [view diff] case mismatch in snippet view article

production. In 1992 she wrote a book with Robert Possee, The Baculovirus Expression Vector System: A Laboratory Guide. In 2006 King co-founded Oxford Expression

Geoffrey L. Smith (1,602 words) [view diff] exact match in snippet view article find links to article

Institutes of Health, USA (1981–84) he developed Vaccinia virus as an expression vector and pioneered the use of genetically engineered viruses as live vaccines

Interleukin (4,194 words) [view diff] exact match in snippet view article find links to article

N, Lee F, Rennick D, Mosmann T, Arai K (Jan 1985). "Use of a cDNA expression vector for isolation of mouse interleukin 2 cDNA clones: expression of T-cell

Selection and amplification binding assay (814 words) [view diff] exact match in snippet view article find links to article

and XhoI and cloned into the SmaI and XhoI restriction site of the expression vector pGEMEXxBal. The recombinant plasmids were transformed into competent

COPS6 (1,332 words) [view diff] exact match in snippet view article find links to article

PMID 10958988. Ramanathan MP, Ayyavoo V, Weiner DB (2001). "Choice of expression vector alters the localization of a human cellular protein". DNA Cell Biol

Coronavirus (10,566 words) [view diff] exact match in snippet view article find links to article

"Engineering the transmissible gastroenteritis virus genome as an expression vector inducing lactogenic immunity". Journal of Virology. 77 (7): 4357–69

Exosome component 5 (971 words) [view diff] exact match in snippet view article find links to article

dendritic cells with CML28 DNA vaccine and SOCS1 small interfering RNA expression vector". Biochemical and Biophysical Research Communications. 347 (1): 200–207

Immunoglobulin heavy constant alpha 1 (1,594 words) [view diff] exact match in snippet view article find links to article

Casadevall A, Green NS (2001). "Human and murine immunoglobulin expression vector cassettes". Mol. Immunol. 37 (14): 837–45. doi:10.1016/S0161-5890(00)00101-2

BacMam (1,671 words) [view diff] exact match in snippet view article find links to article

human beta interferon in insect cells infected with a baculovirus expression vector". Molecular and Cellular Biology. 3 (12): 2156–65. doi:10.1128/MCB

Phage display (4,622 words) [view diff] exact match in snippet view article find links to article

Dübel S, Seehaus T, Klewinghaus I, Little M (August 1991). "A surface expression vector for antibody screening". Gene. 104 (2): 147–53. doi:10.1016/0378-1119(91)90244-6

His-tag (2,639 words) [view diff] exact match in snippet view article find links to article

the forward primer. After PCR amplification, the tag is present on the N-terminus of the gene, which can then be sub-cloned into an expression vector.

MiR-122 (5,471 words) [view diff] exact match in snippet view article find links to article

"[Construction and identification of the human liver-specific miR-122 expression vector]". Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology

Toll-like receptor (6,685 words) [view diff] exact match in snippet view article find links to article

required for full induction of target gene expression by the retrovirus expression vector. This retroviral expression of four transcriptional factors (Oct4

Ingo Potrykus (1,361 words) [view diff] exact match in snippet view article find links to article

resistance genes after protoplast transformation with a new plant expression vector. Nucleic Acids Res. 14:5857-5868. Potrykus I (2001) Golden Rice and

Phycocyanin (3,824 words) [view diff] exact match in snippet view article find links to article

cyanobacterial holo-alpha-phycocyanin in Escherichia coli by using one expression vector". Applied Biochemistry and Biotechnology. 142 (1): 52–9. doi:10

Stefan Dübel (1,026 words) [view diff] exact match in snippet view article find links to article

Thomas; Klewinghaus, Iris; Little, Melvyn (August 1991). "A surface expression vector for antibody screening". Gene. 104 (2): 147–153. doi:10.1016/0378-1119(91)90244-6

Transient expression (1,845 words) [view diff] exact match in snippet view article find links to article

transfected with the polyomavirus large T antigen gene and a DNA expression vector encoding polyomavirus origin (PyOri) for autonomous replication and

Michael Goodin (1,130 words) [view diff] exact match in snippet view article find links to article

Ralf Dietzgen, Goodin invented an Agrobacterium-mediated protein expression vector, pGD, which could generate large amounts of expressed proteins in

Protein engineering (7,502 words) [view diff] exact match in snippet view article find links to article

Next, the treated gene is isolated and cloning into an untreated expression vector in order to prevent mutations in the plasmid backbone.[page needed]

Ectoderm specification (2,122 words) [view diff] exact match in snippet view article find links to article

of a cDNA library from animal caps at a stage of 11.5, cloned into expression vector and generated mRNA. The synthetic RNA was then injected into embryos

Porcine epidemic diarrhoea (2,665 words) [view diff] exact match in snippet view article find links to article

inserting the core neutralizing antigen gene sequence into an appropriate expression vector happens immediately after. However, the bivalent DNA vaccine generated

Gene co-expression network (2,647 words) [view diff] exact match in snippet view article find links to article

correlation calculated for the ranks of gene expression values in a gene expression vector. Several other measures such as partial correlation, regression, and

Anti-immunoglobulin (1,724 words) [view diff] exact match in snippet view article find links to article

inserting genes for an antibody's light and heavy region into an expression vector. The vector is then introduced into host cells for expression. Hosts

Ashwani Pareek (1,108 words) [view diff] exact match in snippet view article find links to article

P1-0019555 //28 June 2018 A method for cloning a hybrid-type histidine kinase gene and an expression vector comprising the hybrid-type histidine kinase gene

Zinc finger chimera (4,966 words) [view diff] exact match in snippet view article find links to article

its sequence is subcloned from pComb3H into a modified bacterial expression vector, pMal-c2, linking it to a sequence coding the maltose binding protein

William A. Haseltine (12,570 words) [view diff] case mismatch in snippet view article find links to article

D; Doetsch PW; Haseltine WA (1984). "Construction of a Mammalian Expression Vector for the E. coli uvrA, b, and C Genes". Plasmid. 11 (3): 253–259. doi:10

Bimolecular fluorescence complementation (5,192 words) [view diff] exact match in snippet view article find links to article

while YFP is used in mammalian cells. Stable cell lines with the expression vector integrated into its genome allows more stable gene expression in the

Benjamin Weiss (scientist) (3,164 words) [view diff] case mismatch in snippet view article

Long-Wu; Davidkova, Genoveva (June 1998). "D2 Dopamine Antisense RNA Expression Vector, Unlike Haloperidol, Produces Long-term Inhibition of D2Dopamine-Mediated

Spinocerebellar ataxia type 1 (10,375 words) [view diff] exact match in snippet view article find links to article

Davidson BL (March 2014). "Broad therapeutic benefit after RNAi expression vector delivery to deep cerebellar nuclei: implications for spinocerebellar