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searching for Buffer solution 46 found (141 total)

Capillary electrophoresis (4,675 words) [view diff] exact match in snippet view article find links to article

vial and capillary are filled with an electrolyte such as an aqueous buffer solution. To introduce the sample, the capillary inlet is placed into a vial

Buffered charcoal yeast extract agar (755 words) [view diff] exact match in snippet view article find links to article

50 °C water bath. Slowly, add enough 1.0 N KOH (about 40 mL) to the buffer solution to bring the pH up to 6.9 and mix. (Do not use NaOH because it has

Buffer P2 (118 words) [view diff] exact match in snippet view article find links to article

Buffer P2 is a lysis buffer solution produced by Qiagen. It contains 1% sodium dodecyl sulfate (SDS) (w/v) to puncture holes in cellular membranes, and

Producer–consumer problem (2,183 words) [view diff] exact match in snippet view article find links to article

synchronization events are exchanges of message." The original semaphore bounded buffer solution was written in ALGOL style. The buffer can store N portions or elements

Lysis buffer (1,825 words) [view diff] exact match in snippet view article find links to article

A lysis buffer is a buffer solution used for the purpose of breaking open cells for use in molecular biology experiments that analyze the labile macromolecules

Protopanaxatriol (222 words) [view diff] exact match in snippet view article find links to article

acid, showing 40% degradation after 4 hours at 37°C both in pH 1.2 buffer solution and rat stomach contents. It is extensively metabolized in mice. Panaxatriol

Nitrogen tribromide (383 words) [view diff] exact match in snippet view article find links to article

reaction of bromine or hypobromite and ammonia in a dilute aqueous buffer solution. It can also be prepared by the reaction of bromine and bromine azide

Moving-boundary electrophoresis (266 words) [view diff] exact match in snippet view article find links to article

moving-boundary electrophoresis apparatus includes a U-shaped cell filled with buffer solution and electrodes immersed at its ends. The sample applied could be any

Bioburden (719 words) [view diff] case mismatch in snippet view article find links to article

dissolved in "Buffered Sodium Chloride-Peptone Solution pH 7.0, Phosphate Buffer Solution pH 7.2, or Soybean-Casein Digest Broth." The Membrane-Filtration Method

Conformational change (1,154 words) [view diff] exact match in snippet view article find links to article

placed on top of short DNA molecules which are then dragged through a buffer solution by application of alternating electrical potentials. By measuring their

Electroblotting (395 words) [view diff] exact match in snippet view article find links to article

onto a membrane. This technique relies upon current and a transfer buffer solution to drive proteins or nucleic acids onto a membrane. Following electrophoresis

DNA separation by silica adsorption (453 words) [view diff] exact match in snippet view article find links to article

The highest DNA adsorption efficiencies occur in the presence of buffer solution with a pH at or below the pKa of the surface silanol groups. The mechanism

Electroantennography (335 words) [view diff] exact match in snippet view article find links to article

ground wire (silver/silver chloride) or a glass electrode filled with a buffer solution to some part of the body, usually inserted into an eye, and another

Interferon alfa (663 words) [view diff] exact match in snippet view article find links to article

milliliter, and the drug product is formulated in isotonic phosphate buffer solution at pH = 7.2, and supplemented with human albumin at 1.5 mg/mL. The

Isoelectric focusing (1,187 words) [view diff] exact match in snippet view article find links to article

a pH gradient is effectively established inside the capillary. The buffer solution in each vessel has an electrical contact with a voltage divider connected

Restriction digest (784 words) [view diff] exact match in snippet view article find links to article

manufacturers that produce such enzymes will often provide a specific buffer solution that contains the unique mix of cations and other components that aid

Capillary electrochromatography (924 words) [view diff] exact match in snippet view article find links to article

vial and capillary are filled with an electrolyte such as an aqueous buffer solution. The capillary is packed with stationary phase. To introduce the sample

Sodium formate (1,044 words) [view diff] exact match in snippet view article find links to article

A solution of formic acid and sodium formate can thus be used as a buffer solution. Sodium formate is slightly water-hazardous and inhibits some species

Food testing strips (354 words) [view diff] exact match in snippet view article find links to article

illness in humans. The enzyme reactant test strips react when the buffer solution breaks the bacterial wall. This breach releases enzymes, which react

Complexometric titration (679 words) [view diff] exact match in snippet view article find links to article

+ 2H+ lies far to the right. Carrying out the reaction in a basic buffer solution removes H+ as it is formed, which also favors the formation of the

Electrolyte (3,486 words) [view diff] exact match in snippet view article find links to article

diffusion of electrolytes with incomplete dissociation. Diffusion in a buffer solution". The Journal of Physical Chemistry. 85 (12): 1756–1762. doi:10.1021/j150612a033

Immunohistochemistry (3,591 words) [view diff] exact match in snippet view article find links to article

is by using high-temperature heating while soaking the slides in a buffer solution. This can be done in different ways, for example by using microwave

Vesicle (biology and chemistry) (3,676 words) [view diff] exact match in snippet view article

or by rapid injection of a phospholipid solution into an aqueous buffer solution. In this way, aqueous vesicle solutions can be prepared of different

Agarose (2,443 words) [view diff] exact match in snippet view article find links to article

cast in a mold, and when set, usually run horizontally submerged in a buffer solution. Tris-acetate-EDTA and Tris-Borate-EDTA buffers are commonly used,

Gibbs–Donnan effect (1,227 words) [view diff] exact match in snippet view article find links to article

groups of the membrane is different from the pH of the rest of the buffer solution. When the charged groups are negative (basic), then they will attract

Antigen retrieval (1,183 words) [view diff] exact match in snippet view article find links to article

the heating conditions. The chemical composition and pH value of the buffer solution also contribute to the effectiveness of heat-induced antigen retrieval

Capillary electrophoresis–mass spectrometry (3,081 words) [view diff] exact match in snippet view article find links to article

Another key factor for successful CE–MS interface is the choice of buffer solution which must be suitable for both CE separation and ESI operation. At

DNA microarray (5,404 words) [view diff] exact match in snippet view article find links to article

bases. The aaDNA is then purified with a column (using a phosphate buffer solution, as Tris contains amine groups). The aminoallyl group is an amine group

Microfluidic modulation spectroscopy (1,410 words) [view diff] no match in snippet view article find links to article

techniques. In microfluidic modulation spectroscopy, the sample (protein-in-buffer) solution and a matching buffer reference stream are introduced into the transmission

Surface plasmon resonance microscopy (7,726 words) [view diff] exact match in snippet view article find links to article

serum albumin (α-RSA). After placing biochip in the SPRi and running buffer solution in the flow cell, α-LAG3 was injected. Special image station was used

Reverse transcription polymerase chain reaction (5,776 words) [view diff] exact match in snippet view article find links to article

mix includes dNTPs, primers, template RNA, necessary enzymes, and a buffer solution. The reaction mix is added to a PCR tube for each reaction, followed

DNA origami (3,574 words) [view diff] exact match in snippet view article find links to article

synthesis. Finally, the scaffold strand and staple strands are mixed in a buffer solution and subjected to a specific temperature cycle. This cycle allows the

Kompetitive allele specific PCR (988 words) [view diff] exact match in snippet view article find links to article

The DNA sample is purified by adding a mixture of chemicals to the buffer solution. In the first round of PCR, a KASP primer mix that contains the two

Aline Miller (1,228 words) [view diff] exact match in snippet view article find links to article

poly(lactic-co-glycolic) acid block copolymer cast films in phosphate buffer solution". Polymer Degradation and Stability. 93 (10): 1869–1876. doi:10.1016/j

CUT&RUN sequencing (2,049 words) [view diff] exact match in snippet view article find links to article

to isolate the nuclei. The nuclei are then centrifuged, washed in a buffer solution, complexed with lectin-coated magnetic beads. The Lectin-Nuclei complex

Bioaerosol (4,171 words) [view diff] exact match in snippet view article find links to article

impact bioaerosols into liquids, such as deionized water or phosphate buffer solution. Collection efficiencies of impingers are shown by Ehrlich et al. (1966)

Forensic biology (6,017 words) [view diff] exact match in snippet view article find links to article

forward and reverse primers, DNA polymerase [usually Taq], dNTPs, and a buffer solution containing Mg2+), qPCR reactions involve fluorescent dye-labelled probes

Mechanosensitive channels (7,825 words) [view diff] exact match in snippet view article find links to article

conductance mechanosensitive channel. The main conductance is 1nS in buffer solution. Channel-proteins have been found in gram-positive and gram-negative

Synchronous coefficient of drag alteration (2,152 words) [view diff] exact match in snippet view article find links to article

the SCODA driving electrodes. Initially the sample is located in a buffer solution in the sample chamber, adjacent to the concentration gel. Injection

Acremonium strictum (3,161 words) [view diff] exact match in snippet view article find links to article

several hours and is converted to oxidized Mn(II). Deaeration of the buffer solution with N2 gas purging suppressed Mn(II) conversion, but this suppression

Colloidal gold (9,821 words) [view diff] exact match in snippet view article find links to article

of gold nanoparticles (AuNPs). Dissolving H 2S into a weak alkaline buffer solution leads to the formation of HS-, which can stabilize AuNPs and ensure

Ethanol-induced non-lamellar phases in phospholipids (4,689 words) [view diff] exact match in snippet view article find links to article

(0–30 mol %) plus a constant concentration of ethanol (5% v/v). An aqueous buffer solution contains the 5% ethanol (v/v) which is desired but due to evaporation

Extremophiles in biotechnology (2,276 words) [view diff] exact match in snippet view article find links to article

tube along with DNA primers, DNA nucleotides, Taq polymerase, and a buffer solution. Once these five key parts are combined they can be put into a PCR

Metal–organic framework (22,767 words) [view diff] exact match in snippet view article find links to article

spectroscopy. Although low quantum yields persist in water and Hepes buffer solution, the luminescence intensity is still strong enough to image cellular

Selective organ targeting (2,174 words) [view diff] exact match in snippet view article find links to article

mix both solutions: the lipid solution containing all lipids and the buffer solution containing mRNA. By employing high-speed mixing, the environmental

Minusheet perfusion culture system (2,555 words) [view diff] exact match in snippet view article find links to article

be easily determined by admixing increasing amounts of biological buffer solution to an aliquot of medium. Then the medium must equilibrate over night