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searching for Lysis buffer 10 found (22 total)

Truenat (825 words) [view diff] exact match in snippet view article find links to article

within one hour from sample collection. Samples are collected in a viral lysis buffer that require very minimal biosafety and biosecurity. The Lancet remarked

Template-switching polymerase chain reaction (441 words) [view diff] exact match in snippet view article find links to article

possible to isolate individual cells and beads together in droplets of lysis buffer, where the polyadenylation site binds to a primer containing a unique

Protein purification (3,910 words) [view diff] exact match in snippet view article find links to article

Alternatively, one or more protease inhibitors can be added to the lysis buffer immediately before cell disruption. Sometimes it is also necessary to

VINE-seq (1,912 words) [view diff] exact match in snippet view article find links to article

separate vascular cells from nuclei and are resuspended in a solution of lysis buffer, RNase inhibitor, and EDTA-free protease inhibitor. The nuclei are then

Western blot (8,047 words) [view diff] exact match in snippet view article find links to article

and releasing the intracellular components. Besides that, the ideal lysis buffer is needed to acquire substantial amounts of target protein content because

Extrachromosomal circular DNA (4,017 words) [view diff] exact match in snippet view article find links to article

crude extrachromosomal DNA, the cells were lysed with a pH 11.8 alkaline lysis buffer, neutralized with a neutralization buffer, and precipitated using a precipitation

Reverse phase protein lysate microarray (2,925 words) [view diff] exact match in snippet view article find links to article

cells collected through any of the above means are lysed with a cell lysis buffer to obtain high protein concentration. Aliquots of the lysates are pooled

NOMe-seq (2,009 words) [view diff] exact match in snippet view article find links to article

trypsin or dispase (depending on cell type), trypan blue, hemocytometer, lysis buffer, and wash buffer To treat nuclei with M.CviPl, components include GpC

Muse cell (8,779 words) [view diff] exact match in snippet view article find links to article

the pellet is re-suspended in PBS and incubated with a red blood cell lysis buffer. Muse cells isolated by this method have been found to be distinct population

Spatial transcriptomics (6,510 words) [view diff] exact match in snippet view article find links to article

laser capture microdissection, where cells are collected directly into lysis buffer and cDNA is generated without the need for RNA isolation, which both