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searching for Transformation efficiency 25 found (33 total)

alternate case: transformation efficiency

Super Optimal Broth (398 words) [view diff] exact match in snippet view article find links to article

used to regenerate Klebsiella oxytoca strains for the improved transformation efficiency. Super Optimal broth with Catabolite repression (SOC) is SOB with
DH5-Alpha Cell (260 words) [view diff] exact match in snippet view article find links to article
engineered by American biologist Douglas Hanahan to maximize transformation efficiency. They are defined by three mutations: recA1, endA1 which help
Nelson Mandela Bay elections (729 words) [view diff] case mismatch in snippet view article find links to article
councillors from the DA. The single councillor of the African Transformation Efficiency and Affirmative Movement crossed to the Liberal Party. The following
Delitto perfetto (1,545 words) [view diff] exact match in snippet view article find links to article
mutation is desired. Longer oligonucleotides lead to increased transformation efficiency. Fully complementary oligonucleotides pairs lead to 5–10 fold
Ribosome display (966 words) [view diff] exact match in snippet view article find links to article
technologies. First, the diversity of the library is not limited by the transformation efficiency of bacterial cells, but only by the number of ribosomes and different
Acetosyringone (668 words) [view diff] exact match in snippet view article find links to article
phytochemicals exuded by plant wounds. This compound also allows higher transformation efficiency in plants, as shown in A. tumefaciens-mediated transformation
Energy quality (2,160 words) [view diff] exact match in snippet view article find links to article
"relative ease" of an energy conversion is only partly dependent on transformation efficiency. As Ohta wrote, "the turbine generator and the electric motor
Spheroplast (1,333 words) [view diff] exact match in snippet view article find links to article
Shergill, K. (2006). "The effect of spheroplast formation on the transformation efficiency in Escherichia coli DH5α" (PDF). Journal of Experimental Microbiology
Gene delivery (2,878 words) [view diff] exact match in snippet view article find links to article
plasmid DNA. Due to the damage caused to the cells and DNA the transformation efficiency of biolistics and electroporation is lower than agrobacterial
Coprinopsis cinerea (1,455 words) [view diff] exact match in snippet view article find links to article
and with small homologous regions can be used, likely with low transformation efficiency. Earlier, REMI (restriction enzyme-mediated integration) could
Bacillus subtilis (6,270 words) [view diff] exact match in snippet view article find links to article
Marburg strain and is widely used in research due to its high transformation efficiency. Bacillus globigii, a closely related but phylogenetically distinct
MRNA display (2,214 words) [view diff] exact match in snippet view article find links to article
size for these three in vivo display systems is limited by the transformation efficiency of each organism. For example, the library size for phage and
Directed evolution (4,761 words) [view diff] exact match in snippet view article find links to article
Such systems are generally only limited in throughput by the transformation efficiency of cells. They are also less expensive and labour-intensive than
Xiphinema americanum (2,716 words) [view diff] exact match in snippet view article find links to article
account for the differences in the experimentally determined transformation efficiency between TomRSV (100%), and TRSV (75% or less). Previous work attempting
Escherichia coli BL21(DE3) (577 words) [view diff] exact match in snippet view article
(hsdR) and modification (hsdM) domains are inactive. This enhances transformation efficiency since exogenously introduced unmethylated DNA remains untargeted
Bacterial one-hybrid system (1,925 words) [view diff] exact match in snippet view article find links to article
system over the yeast one-hybrid system (Y1H) lies in the higher transformation efficiency of plasmids into bacteria which allows for more complex “prey”
Genetic engineering techniques (6,765 words) [view diff] exact match in snippet view article find links to article
plasmid DNA. Due to the damage caused to the cells and DNA the transformation efficiency of biolistics and electroporation is lower than agrobacterial
Genetically modified organism (24,627 words) [view diff] exact match in snippet view article find links to article
Mobasheri H (December 2016). "Impact of heat shock step on bacterial transformation efficiency". Molecular Biology Research Communications. 5 (4): 257–261. PMC 5326489
Cunninghamella elegans (3,012 words) [view diff] exact match in snippet view article find links to article
C. E. (1996). "A method for the large scale isolation of high transformation efficiency fungal genomic DNA". FEMS Microbiology Letters. 145 (2): 261–265
Two-hybrid screening (6,112 words) [view diff] exact match in snippet view article find links to article
advantages over yeast-based systems. For instance, the higher transformation efficiency and faster rate of growth lends E. coli to the use of larger libraries
Artificial gene synthesis (7,073 words) [view diff] exact match in snippet view article find links to article
while the amplified insert assembly seeks to overcome the low transformation efficiency seen in 3A assembly. The BioBrick assembly standard has also served
Naturally occurring phenols (7,616 words) [view diff] exact match in snippet view article find links to article
phytochemicals exuded by plant wounds. This compound also allows higher transformation efficiency in plants, in A. tumefaciens mediated transformation procedures
Dynamic energy budget theory (4,605 words) [view diff] exact match in snippet view article find links to article
proportional to the surface area; food handling time and the transformation efficiency from food to reserve are independent of food density. A fixed
Transplastomic plant (3,091 words) [view diff] exact match in snippet view article find links to article
stable insertion of the gene cassette into the plastid. Whilst the transformation efficiency is lower than in agrobacterial mediated transformation, which
Genome-wide CRISPR-Cas9 knockout screens (6,172 words) [view diff] exact match in snippet view article find links to article
sufficient amounts of high-quality lentivirus; (v) overcoming low transformation efficiency; (vi) proper scaling of the bacterial culture. One of the largest