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searching for Taq polymerase 18 found (69 total)

TOPO cloning (568 words) [view diff] exact match in snippet view article find links to article

cloned into specific vectors without the requirement for DNA ligases. Taq polymerase has a nontemplate-dependent terminal transferase activity that adds

Dark quencher (660 words) [view diff] exact match in snippet view article find links to article

with a specific 5' tail binds to the sequence to be probed, and the Taq polymerase extends the sequence that will have a specific 5' end dependent on the

Extremophiles in biotechnology (2,276 words) [view diff] case mismatch in snippet view article find links to article

various biotechnical processes. A good example of this would be how Taq Polymerase was isolated from the bacteria Thermus aquaticus and was then used to

David Callaway (739 words) [view diff] exact match in snippet view article find links to article

Richter, D.; Callaway, D. J. E. (2005). "Coupled protein domain motion in Taq polymerase revealed by neutron spin-echo spectroscopy". Proc Natl Acad Sci USA

Loop-mediated isothermal amplification (3,191 words) [view diff] exact match in snippet view article find links to article

(typically Bst – Bacillus stearothermophilus – DNA polymerase rather than Taq polymerase as in PCR). Several reports describe successful detection of pathogens

Molecular genetics (3,815 words) [view diff] exact match in snippet view article find links to article

genotypic sequences to phenotypes. Polymerase chain reaction (PCR) using Taq polymerase, invented by Mullis in 1985, enabled scientists to create millions of

Protein dynamics (2,604 words) [view diff] exact match in snippet view article find links to article

Richter D, Callaway DJ (Dec 2005). "Coupled protein domain motion in Taq polymerase revealed by neutron spin-echo spectroscopy" (PDF). Proceedings of the

H.M. Krishna Murthy (906 words) [view diff] case mismatch in snippet view article find links to article

Specifically, Murthy tried to pass some elements of a known structure of TAQ polymerase (PDB entry 1TAQ) as a new structure (PDB entry 1CMW). When the misconduct

Protein engineering (7,486 words) [view diff] exact match in snippet view article find links to article

Increase concentration of Taq polymerase. Increase extension time. Increase cycle time. Use less accurate Taq polymerase. Also see polymerase chain reaction

DNA footprinting (2,156 words) [view diff] case mismatch in snippet view article find links to article

"An Improved Method for Photofootprinting Yeast Genes In Vivo Using Taq Polymerase". Nucleic Acids Res. 17 (1): 171–183. doi:10.1093/NAR/17.1.171. PMC 331543

Mechanotransduction (3,046 words) [view diff] exact match in snippet view article find links to article

Richter D, Callaway DJ (Dec 2005). "Coupled protein domain motion in Taq polymerase revealed by neutron spin-echo spectroscopy". Proceedings of the National

Φ29 DNA polymerase (3,131 words) [view diff] exact match in snippet view article find links to article

is believed to be 1 or 2 orders of magnitude less error prone than Taq polymerase. Generates large fragments, over 10kb. Produces more DNA than PCR-based

Nucleic acid analogue (5,299 words) [view diff] exact match in snippet view article find links to article

of the nucleotides linked to bulky adducts such as florophores by [Taq polymerase]s, the sequence is typically copied using a nucleotide with an arm and

Protein domain (8,443 words) [view diff] exact match in snippet view article find links to article

Richter D, Callaway DJ (December 2005). "Coupled protein domain motion in Taq polymerase revealed by neutron spin-echo spectroscopy". Proceedings of the National

Biological small-angle scattering (3,450 words) [view diff] exact match in snippet view article find links to article

(September 2004). "Structure-specific DNA-induced conformational changes in Taq polymerase revealed by small angle neutron scattering". The Journal of Biological

DNA profiling (11,497 words) [view diff] exact match in snippet view article find links to article

hydrogen bonding. 3-Extension : A thermostable DNA polymerase which is Taq polymerase is commonly used at this step. This is done at a temperature of 72 °C

SNP genotyping (5,012 words) [view diff] exact match in snippet view article find links to article

target DNA strand and then get degraded by 5’-nuclease activity of the Taq polymerase as it extends the DNA from the PCR primers. The degradation of the probe

Yellowstone National Park (16,348 words) [view diff] exact match in snippet view article find links to article

found in the Yellowstone hot springs that produces an important enzyme (Taq polymerase) that is easily replicated in the lab and is useful in replicating DNA