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searching for Selectable marker 36 found (76 total)

Gene gun (2,156 words) [view diff] exact match in snippet view article find links to article

encoding a selectable marker is a common element within DNA constructs and is used to select for properly transformed cells. The selectable marker chosen

Yeast artificial chromosome (1,982 words) [view diff] exact match in snippet view article find links to article

centromere [3], and telomeres [4] from S. cerevisiae. Additionally, selectable marker genes, such as antibiotic resistance and a visible marker, are utilized

Azospirillum brasilense (762 words) [view diff] exact match in snippet view article find links to article

spectinomycin and ampicillin antibiotics. Kanamycin resistance is used as a selectable marker. A. brasilense has a high evolutionary adaptation rate driven by codon

PlasMapper (331 words) [view diff] exact match in snippet view article find links to article

cloning sites, restriction sites, reporter genes, affinity tags, selectable marker genes, origins of replication and open reading frames. PlasMapper

Gene trapping (786 words) [view diff] exact match in snippet view article find links to article

upstream of the insertion site is spliced in frame to the reporter/selectable marker gene. Since transcription is terminated prematurely at the inserted

EHA101 (607 words) [view diff] exact match in snippet view article find links to article

has been useful to plant biotechnologists who use kanamycin as a selectable marker on their binary plasmids. Sciaky, D.; Montoya, A.L. & Chilton, M.-D

Backcrossing (849 words) [view diff] exact match in snippet view article find links to article

a gene (i.e. a knockout), indicated by the presence of a positive selectable marker) is crossed with a mouse of a constant genetic background, the average

URA3 (284 words) [view diff] exact match in snippet view article find links to article

genetic markers in yeast genetic modification. While URA3 is a powerful selectable marker, it has a high background. This background is because cells that pick

Nourseothricin (358 words) [view diff] exact match in snippet view article find links to article

Shen J, Guo W, Köhler JR (2005). "CaNAT1, a heterologous dominant selectable marker for transformation of Candida albicans and other pathogenic Candida

PGLO (612 words) [view diff] exact match in snippet view article find links to article

Alba. Other features on pGLO, like most other plasmids, include a selectable marker and an MCS (multiple cloning site) located at the end of the GFP gene

Robert Fraley (580 words) [view diff] exact match in snippet view article find links to article

able to use Agrobacterium tumefaciens to transfer genes conferring a selectable marker into the cells of petunia plants. Together with Stephen Rogers and

Darrick Carter (1,468 words) [view diff] exact match in snippet view article find links to article

hypoxanthine-xanthine-guanine phosphoribosyltransferase gene as a selectable marker in the context of stable transformation. In early 2000s, he demonstrated

Eliezer Huberman (1,567 words) [view diff] exact match in snippet view article find links to article

inosine 5′-monophosphate dehydrogenase ("IMPDH") DNA as a dominant selectable marker in mammals and other eukaryotes". lens.org. Retrieved 12 November

Michael W. Bevan (821 words) [view diff] exact match in snippet view article find links to article

Chilton, MD (1983). "A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation". Nature. 304 (5922): 184–7. Bibcode:1983Natur

Forward genetics (1,752 words) [view diff] exact match in snippet view article find links to article

modified to transpose only once, and once inserted into the genome a selectable marker can be used to identify the mutagenized individuals. Since a known

Agrobacterium (2,603 words) [view diff] exact match in snippet view article find links to article

contains the T-DNA region of the disarmed plasmid, together with a selectable marker (such as antibiotic resistance) to enable selection for plants that

HaloTag (977 words) [view diff] exact match in snippet view article find links to article

(June 1999). "A bacterial haloalkane dehalogenase gene as a negative selectable marker in Arabidopsis" (PDF). The Plant Journal. 18 (5): 571–6. doi:10.1046/j

Neurogenetics (3,387 words) [view diff] exact match in snippet view article find links to article

The hosts are then screened with the aid of a toxic drug that the selectable marker is resistant to. The use of recombinant DNA is an example of a reverse

Thermotoga maritima (1,971 words) [view diff] exact match in snippet view article find links to article

challenging task primarily because of a lack of a suitable heat-stable selectable marker. Recently, the most reliable genetic system based on pyrimidine biosynthesis

Transfer DNA (1,865 words) [view diff] exact match in snippet view article find links to article

R, Tague BW (2001-12-01). "Phosphomannose isomerase: A versatile selectable marker forArabidopsis thaliana germ-line transformation". Plant Molecular

Termination signal (936 words) [view diff] exact match in snippet view article find links to article

regulation of downstream genes that may be crucial to host cell function. Selectable marker genes that are downstream of the target gene insertion site and genes

Artificial gene synthesis (7,073 words) [view diff] exact match in snippet view article find links to article

destination plasmid, the selectable marker is flanked by outward-cutting BsaI restriction sites. This excises the selectable marker, allowing the insertion

2-Fluoroadenine (683 words) [view diff] exact match in snippet view article find links to article

Dennis A. (1994). "The adenine phosphoribosyltransferase (APRT) selectable marker system". Plant Science. 101 (1). Elsevier BV: 3–9. doi:10.1016/0168-9452(94)90159-7

Genetically modified brinjal (2,948 words) [view diff] exact match in snippet view article find links to article

brinjal's tissue throughout its complete life cycle. NptII and aad are selectable marker genes, nptII is used to identify transgenic plants from non-transgenic

Burkholderia pseudomallei (4,083 words) [view diff] exact match in snippet view article find links to article

"Glyphosate resistance as a novel select-agent-compliant, non-antibiotic-selectable marker in chromosomal mutagenesis of the essential genes asd and dapB of

Chimera (genetics) (6,610 words) [view diff] exact match in snippet view article

Rose SC, Zapata C, Srivatanakul M (1998). "Cross-protection and selectable marker genes in plant transformation". In Vitro Cellular & Developmental

Bacterial one-hybrid system (1,925 words) [view diff] exact match in snippet view article find links to article

required for biosynthesis of histidine and uracil. Using a negative selectable marker is crucial for greatly reducing the incidence of false-positives.

Site-specific recombinase technology (3,200 words) [view diff] exact match in snippet view article find links to article

In the first ("knock-out"-) reaction the gene was tagged with a selectable marker, typically by insertion of a hygtk ([+/-]) cassette providing G418

Pal Maliga (1,330 words) [view diff] exact match in snippet view article find links to article

TN, Svab, Z, Maliga, P (October 2004). "Kanamycin resistance as a selectable marker for plastid transformation in tobacco". Molecular and General Genetics

Jumping library (2,917 words) [view diff] exact match in snippet view article find links to article

construction, and a partial filling-in reaction that removes the need for a selectable marker. This filling-in reaction worked by destroying the specific cohesive

Candida albicans (9,974 words) [view diff] exact match in snippet view article find links to article

Guo W, Köhler JR (February 2005). "CaNAT1, a heterologous dominant selectable marker for transformation of Candida albicans and other pathogenic Candida

Cre-Lox recombination (5,058 words) [view diff] no match in snippet view article find links to article

Michiel (2007). "Cre- lox -Based System for Multiple Gene Deletions and Selectable-Marker Removal in Lactobacillus plantarum". Applied and Environmental Microbiology

Epistasis and functional genomics (1,936 words) [view diff] exact match in snippet view article find links to article

destabilization of mRNA transcripts by integrating an antibiotic selectable marker into the 3’UTR, downstream of the stop codon (figure 2). mRNA’s with

History of genetic engineering (6,419 words) [view diff] exact match in snippet view article find links to article

Chilton, M. D. (1983). "A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation". Nature. 304 (5922): 184–87. Bibcode:1983Natur

Genetically modified crops (16,854 words) [view diff] exact match in snippet view article find links to article

Chilton MD (1983). "A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation. 1983". Biotechnology. 24 (5922): 367–70

Stem cell marker (5,691 words) [view diff] exact match in snippet view article find links to article

Hashiyama M, Suda T, Ozawa K (1999). "Green fluorescent protein as a selectable marker of retrovirally transduced hematopoietic progenitors". Stem Cells