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searching for Primer extension 47 found (59 total)

Small nucleolar RNA SNORA76 (205 words) [view diff] exact match in snippet view article find links to article

its expression in mouse experimentally verified by Northern blot and primer extension analysis. ACA62 is proposed to guide the pseudouridylation of 18S rRNA

Small nucleolar RNA snoR9 plant (235 words) [view diff] exact match in snippet view article find links to article

thaliana by computational screening and experimentally verified by primer extension analysis. This snoRNA is not related to the snoRNA identified in hyperthermophiles

Small nucleolar RNA SNORA79 (267 words) [view diff] exact match in snippet view article find links to article

its expression in mouse experimentally verified by Northern blot and primer extension analysis. It belongs to the H/ACA box class of snoRNAs as it has the

Small nucleolar RNA SNORA77 (267 words) [view diff] exact match in snippet view article find links to article

its expression in mouse experimentally verified by Northern blot and primer extension analysis. It belongs to the H/ACA box class of snoRNAs as it has the

SNP genotyping (5,005 words) [view diff] exact match in snippet view article find links to article

2001). Because primer extension is based on the highly accurate DNA polymerase enzyme, the method is generally very reliable. Primer extension is able to

Bisulfite sequencing (4,084 words) [view diff] exact match in snippet view article find links to article

as a whole rather than at specific CpG sites. MS-SnuPE employs the primer extension method initially designed for analyzing single-nucleotide polymorphisms

Geniom RT Analyzer (900 words) [view diff] case mismatch in snippet view article find links to article

microarray technology in order to quantify microRNAs via a Microfluidic Primer Extension Assay (MPEA) technique (figure 2). Many human diseases such as cancer

Small Cajal body specific RNA 20 (270 words) [view diff] exact match in snippet view article find links to article

its expression in mouse experimentally verified by Northern blot and primer extension analysis. ACA66 is predicted to guide the pseudouridylation of residue

Toeprinting assay (312 words) [view diff] exact match in snippet view article find links to article

The toeprinting assay, also known as the primer extension inhibition assay, is a method used in molecular biology that allows one to examine the interactions

Small Cajal body specific RNA 21 (270 words) [view diff] exact match in snippet view article find links to article

its expression in mouse experimentally verified by Northern blot and primer extension analysis. ACA68 is proposed to guide the pseudouridylation of residue

Site-directed mutagenesis (2,973 words) [view diff] exact match in snippet view article find links to article

approach to site-directed mutagenesis by using oligonucleotides in a primer extension method with DNA polymerase. For his part in the development of this

Cassette mutagenesis (587 words) [view diff] exact match in snippet view article find links to article

directed mutagenesis methods, cassette mutagenesis also does not involve primer extension by DNA polymerase. First, restriction enzymes are used to cleave near

Mutagenesis (molecular biology technique) (2,884 words) [view diff] exact match in snippet view article

primer extension technique developed in 1978. Such techniques commonly involve using pre-fabricated mutagenic oligonucleotides in a primer extension reaction

Thermus thermophilus (1,083 words) [view diff] exact match in snippet view article find links to article

crude samples. It can be used in applications of PCR, RT-PCR and also primer extension. This polymerase has been shown to be resistant to DNA polymerase inhibitors

Aminomethyltransferase (1,436 words) [view diff] exact match in snippet view article find links to article

has a single defined transcription initiation site detected by the primer extension method. Two putative glucocorticoid-responsive elements and a putative

Nucleic acid structure determination (4,451 words) [view diff] exact match in snippet view article find links to article

DNA-protein interactions. Selective 2′-hydroxyl acylation analyzed by primer extension, or SHAPE, takes advantage of reagents that preferentially modify the

Ribozyme (5,181 words) [view diff] exact match in snippet view article find links to article

"RNA-catalyzed RNA polymerization: accurate and general RNA-templated primer extension". Science. 292 (5520): 1319–1325. Bibcode:2001Sci...292.1319J. CiteSeerX 10

Jack W. Szostak (1,517 words) [view diff] case mismatch in snippet view article find links to article

Imidazolium-Bridged Dinucleotide Intermediate in Nonenzymatic RNA Primer Extension". Journal of the American Chemical Society. 138 (36): 11996–12002.

Terminal deoxynucleotidyl transferase (3,518 words) [view diff] exact match in snippet view article find links to article

synthesis of oligonucleotides, with TdT-dNTP tethered analogs capable of primer extension by 1 nt at a time. In other words, the enzyme TdT has demonstrated

Multicopy single-stranded DNA (1,943 words) [view diff] exact match in snippet view article find links to article

digested to release the nascent strand of cDNA. In the case of msDNA primer extension, however, a long strand of RNA remains attached to the 3'-OH of the

Anton Yuryev (2,036 words) [view diff] exact match in snippet view article find links to article

Inform Assoc. 2004 May-Jun;11(3):174-8. Auto-validation of fluorescent primer extension genotyping assay using signal clustering and neural networks Huang

David Bartel (840 words) [view diff] exact match in snippet view article find links to article

"RNA-catalyzed RNA polymerization: accurate and general RNA-templated primer extension". Science. 292 (5520): 1319–25. Bibcode:2001Sci...292.1319J. CiteSeerX 10

DNA sequencing (14,413 words) [view diff] exact match in snippet view article find links to article

method for determining DNA sequences involved a location-specific primer extension strategy established by Ray Wu at Cornell University in 1970. DNA polymerase

Favipiravir (2,962 words) [view diff] exact match in snippet view article find links to article

adenosine for the viral RdRP. Incorporating two such bases in a row stops primer extension, although it is unclear how as of 2013. The US Department of Defense

Polymerase chain reaction optimization (2,029 words) [view diff] exact match in snippet view article find links to article

the 3' end of one primer to itself or the second primer may cause primer extension, resulting in the formation of so-called primer dimers, visible as

ECHS1 (1,259 words) [view diff] exact match in snippet view article find links to article

located 62 and 63 bp upstream of the translation codon, were mapped by primer extension analysis. The 5'-flanking region of the ECHS1 gene is GC-rich and contains

DNA footprinting (2,156 words) [view diff] exact match in snippet view article find links to article

protected and unprotected DNA have been treated, there is subsequent primer extension of the cleaved products. The extension will terminate upon reaching

Ancient DNA (6,202 words) [view diff] exact match in snippet view article find links to article

multi-million year old aDNA would come to be dismissed as inauthentic. Single primer extension amplification was introduced in 2007 to address postmortem DNA modification

RpoS mRNA 5′UTR (337 words) [view diff] case mismatch in snippet view article find links to article

Single Nucleotide Resolution by Selective 2′-Hydroxyl Acylation and Primer Extension (SHAPE)". Journal of the American Chemical Society. 127 (12): 4223–4231

History of Earth (16,266 words) [view diff] case mismatch in snippet view article find links to article

"RNA-Catalyzed RNA Polymerization: Accurate and General RNA-Templated Primer Extension". Science. 292 (5520): 1319–1325. Bibcode:2001Sci...292.1319J. CiteSeerX 10

DNA methylation (13,260 words) [view diff] case mismatch in snippet view article find links to article

from DNA methylation maps. Methylation Sensitive Single Nucleotide Primer Extension Assay (msSNuPE), which uses internal primers annealing straight 5'

Protein engineering (7,479 words) [view diff] exact match in snippet view article find links to article

switching and annealing of the short fragments obtained from the earlier primer extension to other single stranded DNA fragments. This process is repeated until

Twister sister ribozyme (874 words) [view diff] case mismatch in snippet view article find links to article

by conducting SHAPE (Selective-2′ -Hydroxyl Acylation analyzed by Primer Extension) experiments. Weinberg Z, Kim PB, Chen TH, Li S, Harris KA, Lünse CE

DNA damage (naturally occurring) (9,956 words) [view diff] exact match in snippet view article

dimerization (which is indicative of DNA damage) was assessed using sensitive primer extension techniques. It was found that the removal of DNA photolesions was much

SAT1 (gene) (2,126 words) [view diff] exact match in snippet view article

This gene is located on the X chromosome in the region Xp22.1. The primer extension analysis indicated that the transcription started 179 bases upstream

Phosphorimidazolide (872 words) [view diff] case mismatch in snippet view article find links to article

Imidazolium-Bridged Dinucleotide Intermediate in Nonenzymatic RNA Primer Extension". Journal of the American Chemical Society. 138 (36): 11996–12002.

History of life (22,637 words) [view diff] case mismatch in snippet view article find links to article

"RNA-Catalyzed RNA Polymerization: Accurate and General RNA-Templated Primer Extension" (PDF). Science. 292 (5520): 1319–1325. Bibcode:2001Sci...292.1319J

U7 small nuclear RNA (3,628 words) [view diff] exact match in snippet view article find links to article

1016/0167-4781(92)90104-8. PMID 1374647. Kirby BJ, Turner PC (1992). "Primer extension analysis of mouse U7 small nuclear RNA gene expression". Biochem Soc

Ribonucleoprotein Networks Analyzed by Mutational Profiling (1,794 words) [view diff] exact match in snippet view article find links to article

Kevin M. (2015-10-01). "Selective 2′-hydroxyl acylation analyzed by primer extension and mutational profiling (SHAPE-MaP) for direct, versatile and accurate

G-quadruplex (11,903 words) [view diff] exact match in snippet view article find links to article

act as a roadblock and cause polymerase stalling, which halts the primer extension. The dimethyl sulfate (DMS) followed by the piperidine cleavage assay

Hypercycle (chemistry) (7,797 words) [view diff] case mismatch in snippet view article

"RNA-Catalyzed RNA Polymerization: Accurate and General RNA-Templated Primer Extension". Science. 292 (5520): 1319–1325. Bibcode:2001Sci...292.1319J. CiteSeerX 10

Vectorette PCR (2,655 words) [view diff] exact match in snippet view article find links to article

different stages. During the first part, the IP works on amplifying the primer extension while the VP remains hybridized with the product; thus, any background

DNA-encoded chemical library (4,738 words) [view diff] exact match in snippet view article find links to article

covalently attached to the folded yR. (e) The yR is dismantled by primer extension yielding a double-stranded display product exposing the reaction product

Epitranscriptomic sequencing (6,384 words) [view diff] exact match in snippet view article find links to article

conventional base-pairing caused by modifications (ex. m1A, m1G, m22G) RT primer extension at low dNTPs concentration, for mapping of RT arrest signals. Single-molecule

No-SCAR genome editing (5,650 words) [view diff] exact match in snippet view article find links to article

The mismatch between the 3’ end of the primer and wild-type prevents primer extension and thus, only the mutant genotype produces a PCR product. Hotstart

Ancient pathogen genomics (5,940 words) [view diff] exact match in snippet view article find links to article

digestion of environmental microbial DNA using restriction enzymes and primer extension capture (PEC). During the construction of the library, the ssDNA fragments

Non-canonical base pairing (11,728 words) [view diff] exact match in snippet view article find links to article

latest such tools is SHAPE (Selective 2′-hydroxyl acylation analyzed by primer extension), and SHAPE-Directed RNA Secondary Structure Prediction appears to