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Find link is a tool written by Edward Betts.searching for Primer extension 47 found (59 total)
alternate case: primer extension
Small nucleolar RNA SNORA76
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its expression in mouse experimentally verified by Northern blot and primer extension analysis. ACA62 is proposed to guide the pseudouridylation of 18S rRNASmall nucleolar RNA snoR9 plant (235 words) [view diff] exact match in snippet view article find links to article
thaliana by computational screening and experimentally verified by primer extension analysis. This snoRNA is not related to the snoRNA identified in hyperthermophilesSmall nucleolar RNA SNORA79 (267 words) [view diff] exact match in snippet view article find links to article
its expression in mouse experimentally verified by Northern blot and primer extension analysis. It belongs to the H/ACA box class of snoRNAs as it has theSmall nucleolar RNA SNORA77 (267 words) [view diff] exact match in snippet view article find links to article
its expression in mouse experimentally verified by Northern blot and primer extension analysis. It belongs to the H/ACA box class of snoRNAs as it has theSNP genotyping (5,005 words) [view diff] exact match in snippet view article find links to article
2001). Because primer extension is based on the highly accurate DNA polymerase enzyme, the method is generally very reliable. Primer extension is able toBisulfite sequencing (4,084 words) [view diff] exact match in snippet view article find links to article
as a whole rather than at specific CpG sites. MS-SnuPE employs the primer extension method initially designed for analyzing single-nucleotide polymorphismsGeniom RT Analyzer (900 words) [view diff] case mismatch in snippet view article find links to article
microarray technology in order to quantify microRNAs via a Microfluidic Primer Extension Assay (MPEA) technique (figure 2). Many human diseases such as cancerSmall Cajal body specific RNA 20 (270 words) [view diff] exact match in snippet view article find links to article
its expression in mouse experimentally verified by Northern blot and primer extension analysis. ACA66 is predicted to guide the pseudouridylation of residueToeprinting assay (312 words) [view diff] exact match in snippet view article find links to article
The toeprinting assay, also known as the primer extension inhibition assay, is a method used in molecular biology that allows one to examine the interactionsSmall Cajal body specific RNA 21 (270 words) [view diff] exact match in snippet view article find links to article
its expression in mouse experimentally verified by Northern blot and primer extension analysis. ACA68 is proposed to guide the pseudouridylation of residueSite-directed mutagenesis (2,973 words) [view diff] exact match in snippet view article find links to article
approach to site-directed mutagenesis by using oligonucleotides in a primer extension method with DNA polymerase. For his part in the development of thisCassette mutagenesis (587 words) [view diff] exact match in snippet view article find links to article
directed mutagenesis methods, cassette mutagenesis also does not involve primer extension by DNA polymerase. First, restriction enzymes are used to cleave nearMutagenesis (molecular biology technique) (2,884 words) [view diff] exact match in snippet view article
primer extension technique developed in 1978. Such techniques commonly involve using pre-fabricated mutagenic oligonucleotides in a primer extension reactionThermus thermophilus (1,083 words) [view diff] exact match in snippet view article find links to article
crude samples. It can be used in applications of PCR, RT-PCR and also primer extension. This polymerase has been shown to be resistant to DNA polymerase inhibitorsAminomethyltransferase (1,436 words) [view diff] exact match in snippet view article find links to article
has a single defined transcription initiation site detected by the primer extension method. Two putative glucocorticoid-responsive elements and a putativeNucleic acid structure determination (4,451 words) [view diff] exact match in snippet view article find links to article
DNA-protein interactions. Selective 2′-hydroxyl acylation analyzed by primer extension, or SHAPE, takes advantage of reagents that preferentially modify theRibozyme (5,181 words) [view diff] exact match in snippet view article find links to article
"RNA-catalyzed RNA polymerization: accurate and general RNA-templated primer extension". Science. 292 (5520): 1319–1325. Bibcode:2001Sci...292.1319J. CiteSeerX 10Jack W. Szostak (1,517 words) [view diff] case mismatch in snippet view article find links to article
Imidazolium-Bridged Dinucleotide Intermediate in Nonenzymatic RNA Primer Extension". Journal of the American Chemical Society. 138 (36): 11996–12002.Terminal deoxynucleotidyl transferase (3,518 words) [view diff] exact match in snippet view article find links to article
synthesis of oligonucleotides, with TdT-dNTP tethered analogs capable of primer extension by 1 nt at a time. In other words, the enzyme TdT has demonstratedMulticopy single-stranded DNA (1,943 words) [view diff] exact match in snippet view article find links to article
digested to release the nascent strand of cDNA. In the case of msDNA primer extension, however, a long strand of RNA remains attached to the 3'-OH of theAnton Yuryev (2,036 words) [view diff] exact match in snippet view article find links to article
Inform Assoc. 2004 May-Jun;11(3):174-8. Auto-validation of fluorescent primer extension genotyping assay using signal clustering and neural networks HuangDavid Bartel (840 words) [view diff] exact match in snippet view article find links to article
"RNA-catalyzed RNA polymerization: accurate and general RNA-templated primer extension". Science. 292 (5520): 1319–25. Bibcode:2001Sci...292.1319J. CiteSeerX 10DNA sequencing (14,413 words) [view diff] exact match in snippet view article find links to article
method for determining DNA sequences involved a location-specific primer extension strategy established by Ray Wu at Cornell University in 1970. DNA polymeraseFavipiravir (2,962 words) [view diff] exact match in snippet view article find links to article
adenosine for the viral RdRP. Incorporating two such bases in a row stops primer extension, although it is unclear how as of 2013. The US Department of DefensePolymerase chain reaction optimization (2,029 words) [view diff] exact match in snippet view article find links to article
the 3' end of one primer to itself or the second primer may cause primer extension, resulting in the formation of so-called primer dimers, visible asECHS1 (1,259 words) [view diff] exact match in snippet view article find links to article
located 62 and 63 bp upstream of the translation codon, were mapped by primer extension analysis. The 5'-flanking region of the ECHS1 gene is GC-rich and containsDNA footprinting (2,156 words) [view diff] exact match in snippet view article find links to article
protected and unprotected DNA have been treated, there is subsequent primer extension of the cleaved products. The extension will terminate upon reachingAncient DNA (6,202 words) [view diff] exact match in snippet view article find links to article
multi-million year old aDNA would come to be dismissed as inauthentic. Single primer extension amplification was introduced in 2007 to address postmortem DNA modificationRpoS mRNA 5′UTR (337 words) [view diff] case mismatch in snippet view article find links to article
Single Nucleotide Resolution by Selective 2′-Hydroxyl Acylation and Primer Extension (SHAPE)". Journal of the American Chemical Society. 127 (12): 4223–4231History of Earth (16,266 words) [view diff] case mismatch in snippet view article find links to article
"RNA-Catalyzed RNA Polymerization: Accurate and General RNA-Templated Primer Extension". Science. 292 (5520): 1319–1325. Bibcode:2001Sci...292.1319J. CiteSeerX 10DNA methylation (13,260 words) [view diff] case mismatch in snippet view article find links to article
from DNA methylation maps. Methylation Sensitive Single Nucleotide Primer Extension Assay (msSNuPE), which uses internal primers annealing straight 5'Protein engineering (7,479 words) [view diff] exact match in snippet view article find links to article
switching and annealing of the short fragments obtained from the earlier primer extension to other single stranded DNA fragments. This process is repeated untilTwister sister ribozyme (874 words) [view diff] case mismatch in snippet view article find links to article
by conducting SHAPE (Selective-2′ -Hydroxyl Acylation analyzed by Primer Extension) experiments. Weinberg Z, Kim PB, Chen TH, Li S, Harris KA, Lünse CEDNA damage (naturally occurring) (9,956 words) [view diff] exact match in snippet view article
dimerization (which is indicative of DNA damage) was assessed using sensitive primer extension techniques. It was found that the removal of DNA photolesions was muchSAT1 (gene) (2,126 words) [view diff] exact match in snippet view article
This gene is located on the X chromosome in the region Xp22.1. The primer extension analysis indicated that the transcription started 179 bases upstreamPhosphorimidazolide (872 words) [view diff] case mismatch in snippet view article find links to article
Imidazolium-Bridged Dinucleotide Intermediate in Nonenzymatic RNA Primer Extension". Journal of the American Chemical Society. 138 (36): 11996–12002.History of life (22,637 words) [view diff] case mismatch in snippet view article find links to article
"RNA-Catalyzed RNA Polymerization: Accurate and General RNA-Templated Primer Extension" (PDF). Science. 292 (5520): 1319–1325. Bibcode:2001Sci...292.1319JU7 small nuclear RNA (3,628 words) [view diff] exact match in snippet view article find links to article
1016/0167-4781(92)90104-8. PMID 1374647. Kirby BJ, Turner PC (1992). "Primer extension analysis of mouse U7 small nuclear RNA gene expression". Biochem SocRibonucleoprotein Networks Analyzed by Mutational Profiling (1,794 words) [view diff] exact match in snippet view article find links to article
Kevin M. (2015-10-01). "Selective 2′-hydroxyl acylation analyzed by primer extension and mutational profiling (SHAPE-MaP) for direct, versatile and accurateG-quadruplex (11,903 words) [view diff] exact match in snippet view article find links to article
act as a roadblock and cause polymerase stalling, which halts the primer extension. The dimethyl sulfate (DMS) followed by the piperidine cleavage assayHypercycle (chemistry) (7,797 words) [view diff] case mismatch in snippet view article
"RNA-Catalyzed RNA Polymerization: Accurate and General RNA-Templated Primer Extension". Science. 292 (5520): 1319–1325. Bibcode:2001Sci...292.1319J. CiteSeerX 10Vectorette PCR (2,655 words) [view diff] exact match in snippet view article find links to article
different stages. During the first part, the IP works on amplifying the primer extension while the VP remains hybridized with the product; thus, any backgroundDNA-encoded chemical library (4,738 words) [view diff] exact match in snippet view article find links to article
covalently attached to the folded yR. (e) The yR is dismantled by primer extension yielding a double-stranded display product exposing the reaction productEpitranscriptomic sequencing (6,384 words) [view diff] exact match in snippet view article find links to article
conventional base-pairing caused by modifications (ex. m1A, m1G, m22G) RT primer extension at low dNTPs concentration, for mapping of RT arrest signals. Single-moleculeNo-SCAR genome editing (5,650 words) [view diff] exact match in snippet view article find links to article
The mismatch between the 3’ end of the primer and wild-type prevents primer extension and thus, only the mutant genotype produces a PCR product. HotstartAncient pathogen genomics (5,940 words) [view diff] exact match in snippet view article find links to article
digestion of environmental microbial DNA using restriction enzymes and primer extension capture (PEC). During the construction of the library, the ssDNA fragmentsNon-canonical base pairing (11,728 words) [view diff] exact match in snippet view article find links to article
latest such tools is SHAPE (Selective 2′-hydroxyl acylation analyzed by primer extension), and SHAPE-Directed RNA Secondary Structure Prediction appears to