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Longer titles found: Fluorophore-assisted carbohydrate electrophoresis (view)

searching for Fluorophore 95 found (231 total)

Optical brightener (839 words) [view diff] exact match in snippet view article find links to article

reflected by the material, with the blue and purple optical emission of the fluorophore. The most common classes of compounds with this property are the stilbenes

Photoactivated localization microscopy (3,213 words) [view diff] exact match in snippet view article find links to article

of dyes are used for PALM, STORM and related techniques, both organic fluorophores and fluorescent proteins. Some are compatible with live cell imaging

Immunophenotyping (253 words) [view diff] exact match in snippet view article find links to article

needed] Immunophenotyping is a very common flow cytometry test in which fluorophore-conjugated antibodies are used as probes for staining target cells with

Glow stick (3,123 words) [view diff] exact match in snippet view article find links to article

when the second fluorophore would degrade in solution or be attacked by the chemicals. The emission spectrum of the first fluorophore and the absorption

Molecular imaging (2,567 words) [view diff] exact match in snippet view article find links to article

cancers. An NIR fluorophore has been conjugated to epidermal growth factor (EGF) for imaging of tumor progression. An NIR fluorophore was compared to

SNP genotyping (5,012 words) [view diff] exact match in snippet view article find links to article

the probe is a fluorophore and to the other end a fluorescence quencher. Because of the stem-loop structure of the probe, the fluorophore is close to the

Fluorescence interference contrast microscopy (2,776 words) [view diff] exact match in snippet view article find links to article

between the observed fluorescence intensity and the distance of the fluorophore from a reflective silicon surface. The observed fluorescence intensity

Biosensor (12,153 words) [view diff] exact match in snippet view article find links to article

solvatochromic fluorophore, whose emission properties are sensitive to the nature of its local environment, in a single macromolecule. The fluorophore transduces

Schild equation (1,234 words) [view diff] exact match in snippet view article find links to article

fluorescent-labeled ligands have to bear a bulky fluorophore that may cause it to hinder the ligand binding. Therefore, the fluorophore used, the length of the linker,

Ultrasound-switchable fluorescence imaging (1,434 words) [view diff] exact match in snippet view article find links to article

in 2009, he developed an ultrasound-modulated fluorescence based on a fluorophore-quencher-labeled microbubble system which can control the fluorescent

Protein G (549 words) [view diff] exact match in snippet view article find links to article

forms of protein G. This recombinant protein G, either labeled with a fluorophore or a single-stranded DNA strand, was used as a replacement for secondary

Chromoprotein (739 words) [view diff] exact match in snippet view article find links to article

toxic. Color-changing mutagenesis on amino acids 64–65 of the mRFP1 fluorophore was done to acquire different colors. Chromoproteins are valuable in

Protocatechuate 3,4-dioxygenase (352 words) [view diff] exact match in snippet view article find links to article

cofactor, iron. This enzyme has been found effective at improving organic fluorophore-stability in single-molecule experiments. Commercial preps of the enzyme

Nick translation (416 words) [view diff] exact match in snippet view article find links to article

the alpha phosphate position, often using phosphorus-32. Similarly, a fluorophore can be attached instead for fluorescent labelling, or an antigen for

ELISpot (1,980 words) [view diff] exact match in snippet view article find links to article

amplified in the FluoroSpot with the use of fluorophore-labeled anti-tag antibody and streptavidin-fluorophore conjugate. A fluorescence enhancer solution

Thrombodynamics test (663 words) [view diff] exact match in snippet view article find links to article

fluorophore is registered by CCD camera. Mathematical methods are used to restore spatio-temporal distribution of the thrombin from the fluorophore signal

Epicocconone (441 words) [view diff] exact match in snippet view article find links to article

Epicoconone is notable because it the first covalent/reversible/turn-on fluorophore to be discovered and is a natural product with a new fluorescent scaffold

Fluorescent treponemal antibody absorption test (411 words) [view diff] exact match in snippet view article find links to article

antibodies will bind to the bacteria. FITC (a fluorophore)-labeled anti-treponeme antibody and TRITC (another fluorophore)-labeled anti-human antibodies are added

Quenching (fluorescence) (1,023 words) [view diff] exact match in snippet view article

spatial overlap of donor and quencher molecular orbitals. In most donor-fluorophore–quencher-acceptor situations, the Förster mechanism is more important

Phalloidin (1,696 words) [view diff] exact match in snippet view article find links to article

effective visualization of actin in microscopy. Derivatives conjugated with fluorophores are sold widely. Because of its ability to selectively bind filamentous

Minflux (1,842 words) [view diff] exact match in snippet view article find links to article

exactly on the fluorophore, the system records no emission. Thus, the system requires few emitted photons to determine the fluorophore's location with

Peridinin (301 words) [view diff] exact match in snippet view article find links to article

as fluorescence-activated cell sorting (FACS) and flow cytometry. The fluorophore is covalently linked to proteins or antibodies for use in research applications

Microscopy (8,319 words) [view diff] exact match in snippet view article find links to article

combination of antibodies coupled to a fluorophore as in immunostaining. Examples of commonly used fluorophores are fluorescein or rhodamine. The antibodies

Chemical imaging (4,425 words) [view diff] exact match in snippet view article find links to article

PMID 8756483. FLIM | Fluorescence Lifetime Imaging Microscopy: Fluorescence, fluorophore chemical imaging, confocal emission microspectroscopy, FRET, cross-correlation

Fluorescence correlation spectroscopy (7,969 words) [view diff] exact match in snippet view article find links to article

{\displaystyle \langle N\rangle } of fluorophores in the focal volume is low and if dark states, etc., of the fluorophore can be ignored. In particular, no

Illumina dye sequencing (1,984 words) [view diff] exact match in snippet view article find links to article

the DNA strand. Only one base is able to be added per round due to the fluorophore acting as a blocking group; however, the blocking group is reversible

Collagen hybridizing peptide (1,928 words) [view diff] exact match in snippet view article find links to article

collagen types (e.g., Col I, II, IV). Studies also showed CHPs and their fluorophore conjugates have superior stability in contact with serum. Controlled

Small conditional RNA (464 words) [view diff] exact match in snippet view article find links to article

dynamic RNA nanotechnology, molecular programming, and synthetic biology. Fluorophore-labeled scRNAs have been engineered to transduce between detection of

Protein–carbohydrate interaction (760 words) [view diff] exact match in snippet view article find links to article

separation and plenty of ways to get fluorophore source: there are some of amino acids and ligands that have fluorophore after they are activated. Dual polarisation

Sanger sequencing (3,344 words) [view diff] exact match in snippet view article find links to article

means of a fluorophore covalently attached to the oligonucleotide primer used in enzymatic DNA sequence analysis. A different coloured fluorophore is used

Optical sectioning (1,337 words) [view diff] exact match in snippet view article find links to article

and multi-photon excitation techniques take advantage of the fact that fluorophores can be excited not just by a single photon of the correct energy but

Benzoxazole (361 words) [view diff] exact match in snippet view article find links to article

"Photophysical, Electrochemical and Crystallographic Investigations of the Fluorophore 2,5-Bis(5-tert-butyl-benzoxazol-2-yl)thiophene". The Journal of Physical

Benzoxazole (361 words) [view diff] exact match in snippet view article find links to article

"Photophysical, Electrochemical and Crystallographic Investigations of the Fluorophore 2,5-Bis(5-tert-butyl-benzoxazol-2-yl)thiophene". The Journal of Physical

Phycobiliprotein (538 words) [view diff] exact match in snippet view article find links to article

demonstrate superior fluorescent properties compared to small organic fluorophores, especially when high sensitivity or multicolor detection required :

Polony sequencing (1,771 words) [view diff] exact match in snippet view article find links to article

Cy5‐NNNNNNNNT 5' Cy3‐NNNNNNNNA 5' TexasRed‐NNNNNNNNC 5' 6FAM‐NNNNNNNNG The fluorophore-tagged nonamers anneal with differential success to the tag sequences

Affinity electrophoresis (2,124 words) [view diff] exact match in snippet view article find links to article

dissociation constants between molecules. As affinity probes in CAE, fluorophore-labeled compounds with affinities for the target molecules are employed

Eos (protein) (2,618 words) [view diff] exact match in snippet view article

tetrameric, and exhibits strong Forster resonance coupling within individual fluorophores. Like other fluorescent proteins, Eos can be used to report diverse signals

DNA nanoball sequencing (2,006 words) [view diff] exact match in snippet view article find links to article

imaged to determine which nucleotide base bound to the DNA nanoball. The fluorophore is excited with a laser that excites specific wavelengths of light. The

Suliana Manley (1,431 words) [view diff] exact match in snippet view article find links to article

Christian; Manley, Suliana; Johnsson, Kai (2013). "A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins". Nature

Ahmet Yıldız (scientist) (364 words) [view diff] exact match in snippet view article

cited 1427 times."Ahmet Yildiz". "Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm Localization" by Ahmet Yildiz, Joseph N. Forkey,

Anisotropy (2,638 words) [view diff] exact match in snippet view article find links to article

Anisotropy measurements reveal the average angular displacement of the fluorophore that occurs between absorption and subsequent emission of a photon. In

Lanthanide (10,324 words) [view diff] exact match in snippet view article find links to article

negative results. This method involves two fluorophores: a donor and an acceptor. Excitation of the donor fluorophore (in this case, the lanthanide ion complex)

Umbelliferone (989 words) [view diff] exact match in snippet view article find links to article

of a fluorescent hydrogen peroxide probe based on the umbelliferone fluorophore". Tetrahedron Letters. 49 (19): 3045–3048. doi:10.1016/j.tetlet.2008

Detection of genetically modified organisms (1,401 words) [view diff] exact match in snippet view article find links to article

real-time PCR. QRT-PCR methods use fluorescent dyes, such as Sybr Green, or fluorophore-containing DNA probes, such as TaqMan, to measure the amount of amplified

Peter Rutledge (1,220 words) [view diff] exact match in snippet view article find links to article

Price, Jason R.; Todd, Matthew H.; Rutledge, Peter J. (2011). "A Click Fluorophore Sensor that Can Distinguish CuII and HgII via Selective Anion-Induced

Electrophoretic mobility shift assay (1,131 words) [view diff] exact match in snippet view article find links to article

stoichiometry of the protein interaction of interest. Competition between fluorophore- or biotin-labeled probe and unlabeled DNA of the same sequence can be

Erlang distribution (1,949 words) [view diff] exact match in snippet view article find links to article

Selvin, Paul R. (2003-06-27). "Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm Localization". Science. 300 (5628): 2061–2065. doi:10

Cyanine (1,829 words) [view diff] exact match in snippet view article find links to article

nanomol can be detected in gel electrophoresis by naked eye) and its fluorophore emission maximum in the red region, where many CCD detectors have maximum

Dennis A. Dougherty (1,089 words) [view diff] exact match in snippet view article find links to article

Chanda, B.; Bezanilla, F.; Dougherty, A.; Lester, A. (Jul 2004). "A fluorophore attached to nicotinic acetylcholine receptor βM2 detects productive binding

Friedel–Crafts reaction (2,150 words) [view diff] exact match in snippet view article find links to article

anhydride with resorcinol in the presence of zinc chloride gives the fluorophore fluorescein. Replacing resorcinol by N,N-diethylaminophenol in this reaction

Albert Coons (1,142 words) [view diff] exact match in snippet view article find links to article

the war, they had been successful in coupling anthracene isocyanate—a fluorophore—to antipneumococcus antibodies. The resulting product retained its immunological

Neural plate (2,461 words) [view diff] exact match in snippet view article find links to article

contrast to in situ hybridization however, immunofluorescence uses a fluorophore attached to an antibody with biomolecule target, such as proteins, rather

Gregorio Weber (1,699 words) [view diff] exact match in snippet view article find links to article

fluorescence polarization to fluorophores associated with random orientations with ellipsoids of revolution and to mixtures of fluorophores; the first spectral

Malachite green (1,769 words) [view diff] exact match in snippet view article find links to article

protein/fluorogen system. Malachite green is in a class of molecules called fluorophores. When malachite green's rotational freedom is restricted, it transforms

Protein A (1,860 words) [view diff] exact match in snippet view article find links to article

including protein A–gold (PAG) stain is used in immunogold labelling, fluorophore coupled protein A for immunofluorescence, and DNA docking strand coupled

Cellulase (3,191 words) [view diff] exact match in snippet view article find links to article

(β-glucosidase) then acts to hydrolyse the fragment containing the chromophore or fluorophore. The assay is terminated by the addition of a basic solution that stops

Renilla-luciferin 2-monooxygenase (1,267 words) [view diff] exact match in snippet view article find links to article

reaction involving RLuc is passed via resonance energy transfer to the fluorophore of RrGFP and emitted as a green photon (505 nm), resulting in green bioluminescence

Retinal ganglion cell (3,656 words) [view diff] exact match in snippet view article find links to article

through a fluorescence microscope at 50x magnification. The optic nerve was injected with a fluorophore, causing retinal ganglion cells to fluoresce.

Three-photon microscopy (2,109 words) [view diff] exact match in snippet view article find links to article

light and strength of photobleaching. In three-photon excitation, the fluorophore absorbs three photons almost simultaneously. The wavelength of the excitation

Affinity label (1,176 words) [view diff] exact match in snippet view article find links to article

an activity-based probe. The tag may be either a reporter such as a fluorophore or an affinity label such as biotin or an alkyne or azide for use with

Adenylylation (3,274 words) [view diff] exact match in snippet view article find links to article

unrecognized modified protein and label VopS substrates, ATP derivatives with a fluorophore at the adenine N6 NH2 is utilized to do that. Antibody is famous for

Galectin-8 (1,448 words) [view diff] exact match in snippet view article find links to article

progressively acidifying endosomes. Galectin-8 can be tagged with a fluorophore to track these disrupted endosomal membranes, especially when coupled

Henry Colecraft (381 words) [view diff] exact match in snippet view article find links to article

deubiquitinases. These are deubiquitinases where the catalytic domain is fused to fluorophore-specific nanobodies. He is also interested how mutations in ion channels

Lipid bilayer fusion (3,384 words) [view diff] exact match in snippet view article find links to article

Fluorescence quenching assays with ANTS/DPX: ANTS is a polyanionic fluorophore, while DPX is a cationic quencher. The assay is based on the collisional

Microarray analysis techniques (3,559 words) [view diff] exact match in snippet view article find links to article

Depending on the type of array, signal related to nonspecific binding of the fluorophore can be subtracted to achieve better results. One approach involves subtracting

Alexa Fluor (1,102 words) [view diff] exact match in snippet view article find links to article

com. Retrieved 1 March 2020. ThermoFisher Staff (26 February 2020). "Fluorophore Selection". Thermofisher.com. Retrieved 1 March 2020. LifeTechnologies

Microlens (2,098 words) [view diff] exact match in snippet view article find links to article

"Colloidal lenses allow high-temperature single-molecule imaging and improve fluorophore photostability". Nature Nanotechnology. 5 (2): 127–132. Bibcode:2010NatNa

Taekjip Ha (1,711 words) [view diff] exact match in snippet view article find links to article

McKinney, YE Goldman, PR Selvin, Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization, in: Science. Vol. 300, nº 5628; 2061-2065

Riboprobe (1,040 words) [view diff] exact match in snippet view article find links to article

strategy: hapten-modified nucleotides are used in indirect labeling, and fluorophore-modified nucleotides are used in direct labeling. The target DNA and

Western blot (6,081 words) [view diff] exact match in snippet view article find links to article

Another method of secondary antibody detection utilizes a near-infrared fluorophore-linked antibody. The light produced from the excitation of a fluorescent

Ellen Sletten (786 words) [view diff] exact match in snippet view article find links to article

By combining the short-wave infrared (flavylium heterocycle-based) fluorophore with lasers and an appropriate camera, Sletten showed it was possible

Green fluorescent protein (7,449 words) [view diff] exact match in snippet view article find links to article

while in the reaction of 7b to 9 a proton is abstracted. The formed HBI fluorophore is highlighted in green. The reactions are catalyzed by residues Glu222

Immunolabeling (2,922 words) [view diff] exact match in snippet view article find links to article

immuno-fluorescence method, but a colloidal gold tag is used instead of a fluorophore. Overall, the concepts are very parallel in that an unconjugated primary

Lanthanide probes (2,421 words) [view diff] exact match in snippet view article find links to article

technique relies on a distant- dependent transfer of energy from a donor fluorophore to an acceptor dye. Lanthanide probes has been used to study DNA-protein

Emixustat (1,455 words) [view diff] exact match in snippet view article find links to article

Weng J, Travis GH (June 2000). "Biosynthesis of a major lipofuscin fluorophore in mice and humans with ABCR-mediated retinal and macular degeneration"

Glossary of cellular and molecular biology (0–L) (29,380 words) [view diff] exact match in snippet view article

fluorescent labelling, so as to "block" potential off-target binding of the fluorophore to the membrane, which might otherwise cause background fluorescence

Lipid raft (6,661 words) [view diff] exact match in snippet view article find links to article

gain information of fluorophore mobility in the membrane, Fluorescence Resonance Energy Transfer (FRET) can detect when fluorophores are in close proximity

Downhill folding (2,189 words) [view diff] exact match in snippet view article find links to article

associated with unfolding, fluorescence on the immediate environment of the fluorophore, FRET on the average dimensions of the molecule and CD on the secondary

Leptosperin (267 words) [view diff] exact match in snippet view article find links to article

Kerry M. (1 January 2017). "Leptosperin is a distinct and detectable fluorophore in Leptospermum honeys". Food Chemistry. 214: 102–109. doi:10.1016/j

Natalie Banerji (537 words) [view diff] exact match in snippet view article find links to article

(1 July 2006). "Photoproduction of proton gradients with pi-stacked fluorophore scaffolds in lipid bilayers". Science. 313 (5783): 84–86. doi:10.1126/SCIENCE

DNA methylation (13,328 words) [view diff] exact match in snippet view article find links to article

(adenine methylation) GATC sites in an oligonucleotide labeled with a fluorophore and quencher. The adenine methyltransferase methylates the oligonucleotide

Cycloparaphenylene (2,675 words) [view diff] exact match in snippet view article find links to article

"Selective Synthesis of Strained [7]Cycloparaphenylene: An Orange-Emitting Fluorophore". Journal of the American Chemical Society. 133 (40): 15800–15802. doi:10

Trimethylplatinum iodide (1,206 words) [view diff] exact match in snippet view article find links to article

properties of a highly luminescent terpyridine-diphenylacetylene hybrid fluorophore and its metal complexes". Dalton Transactions. 44 (1): 254–267. doi:10

Paul L. Edmiston (1,305 words) [view diff] exact match in snippet view article find links to article

Fluorene Based on the Incorporation of an Environmentally Sensitive Fluorophore Proximal to a Molecularly Imprinted Binding Site, Anal. Chem., 78 (11)

Three-photon adaptive optics microscopy (1,174 words) [view diff] exact match in snippet view article find links to article

three-photon excitation, the target fluorophore absorbs three photons of roughly one-third of the fluorophore's excitation energy almost simultaneously

Sabyasachi Sarkar (1,983 words) [view diff] exact match in snippet view article find links to article

(24 March 2013). "Graphene oxide from silk cocoon: a novel magnetic fluorophore for multi-photon imaging". 3 Biotech. 4 (1): 67–75. doi:10.1007/s13205-013-0128-2

Thermal shift assay (5,887 words) [view diff] exact match in snippet view article find links to article

residues. Benefits include tag-free analysis, avoidance of extrinsic fluorophores, low sample consumption, easy of use, amenity to automation, and high

Spatial transcriptomics (6,588 words) [view diff] exact match in snippet view article find links to article

implemented short (50 base pairs) oligonucleotide probes conjugated with 5 fluorophores which could bind to a specific transcript yielding bright spots in the

O-GlcNAc (12,477 words) [view diff] exact match in snippet view article find links to article

a metabolic chemical reporter that is processed intracellularly to a fluorophore-labeled UDP-GlcNAc analogue, has been shown to achieve one-step fluorescent

Neuronal lineage marker (3,890 words) [view diff] exact match in snippet view article find links to article

behavioral manipulations. Some examples are the use of, digoxigenin- or fluorophore-conjugated oligo- nucleotide probes, for the detection of localized mRNAs

Slot-die coating (4,993 words) [view diff] exact match in snippet view article find links to article

organic light-emitting-diodes using an N-annulated perylene diimide fluorophore". Journal of Materials Chemistry C. 8 (7): 2314–2319. doi:10.1039/C9TC05584C

Upconverting nanoparticles (8,663 words) [view diff] exact match in snippet view article find links to article

nodes and to assist in staging for cancer surgery. UCNPs as targeted fluorophores and conjugated with ligands form over-expressed receptors on malignant

Yoke Khin Yap (1,997 words) [view diff] exact match in snippet view article find links to article

as a fluorophore with enhanced brightness by up to 1000 times, enabling the conversion of existing dye molecules into high-brightness fluorophores (HBFs)

List of protein subcellular localization prediction tools (5,169 words) [view diff] exact match in snippet view article find links to article

designed to perform organelle-based colocalisation analysis from multi-fluorophore microscopy 2D, 3D and 4D cell imaging. (bio.tools entry) http://obcol

Cluster of Excellence Frankfurt Macromolecular Complexes (15,449 words) [view diff] exact match in snippet view article find links to article

(LSFM)). In LSFM, optical sectioning in the excitation process minimizes fluorophore bleaching and phototoxic effects. Because with LSFM biological specimens