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searching for Firefly luciferase 46 found (58 total)

Pyrosequencing (1,110 words) [view diff] exact match in snippet view article find links to article

detection using the firefly luciferase enzyme. A mixture of three enzymes (DNA polymerase, ATP sulfurylase and firefly luciferase) and a nucleotide (dNTP)

Photinini (254 words) [view diff] exact match in snippet view article find links to article

differently colored light and other properties, and in most cases where "firefly luciferase" is used in some application or study, it is the specific luciferase

Bioluminescence imaging (1,585 words) [view diff] exact match in snippet view article find links to article

Systems derived from the three groups above differ in key ways: Firefly luciferase requires D-luciferin to be injected into the subject prior to imaging

Bernard L. Strehler (140 words) [view diff] exact match in snippet view article find links to article

off light after being combined with ATP, magnesium ion, oxygen, and firefly luciferase. In 1956, he switched to the field of aging. He joined the faculty

Jennifer Loros (1,263 words) [view diff] exact match in snippet view article find links to article

firefly luciferase in Neurospora, problematic as firefly luciferase serves as a reporter to measure transcription in cells. By modifying the firefly luciferase

Ataluren (1,637 words) [view diff] exact match in snippet view article find links to article

biological target of ataluren, it was discovered to bind and stabilize firefly luciferase, thus explaining the mechanism by which it created a false positive

Andrew Millar (scientist) (1,052 words) [view diff] exact match in snippet view article

studying plant circadian biology. Millar began experimenting with the firefly luciferase reporter gene as a graduate student at The Rockefeller University

Peroxisome (3,863 words) [view diff] exact match in snippet view article find links to article

used morphological term “microbodies”. Later, it was described that firefly luciferase is targeted to peroxisomes in mammalian cells, allowing the discovery

Southern cotton rat (348 words) [view diff] exact match in snippet view article find links to article

with a bovine adenovirus type 3 recombinant virus expressing the firefly luciferase gene". J. Gen. Virol. 77 (1): 1–9. doi:10.1099/0022-1317-77-1-1. PMID 8558115

Peroxisomal targeting signal (523 words) [view diff] exact match in snippet view article find links to article

"Identification of a peroxisomal targeting signal at the carboxy terminus of firefly luciferase -- Gould et al. 105 (6): 2923 -- The Journal of Cell Biology". www

Drilaster axillaris (155 words) [view diff] exact match in snippet view article find links to article

Mayumi; Shintani, Takeru; Furuhashi, Mana; Inouye, Satoshi (2012). "Firefly luciferase genes from the subfamilies Psilocladinae and Ototretinae (Lampyridae

Kamini Nirmala Mendis (441 words) [view diff] exact match in snippet view article find links to article

D (1993). "Transfection of the malaria parasite and expression of firefly luciferase". Proceedings of the National Academy of Sciences of the United States

Toltec cotton rat (322 words) [view diff] exact match in snippet view article find links to article

with a bovine adenovirus type 3 recombinant virus expressing the firefly luciferase gene". J. Gen. Virol. 77 (1): 1–9. doi:10.1099/0022-1317-77-1-1. PMID 8558115

Photinus pyralis (3,556 words) [view diff] exact match in snippet view article find links to article

375-393 Keller, G.A.; Gould, S.; Deluca, M.; Subramani, S. (1987). "Firefly luciferase is targeted to peroxisomes in mammalian cells". Proceedings of the

Reporter gene (1,980 words) [view diff] exact match in snippet view article find links to article

and chemical biology. Because the reporter enzymes themselves (e.g. firefly luciferase) can be direct targets of small molecules and confound the interpretation

Dyann Wirth (455 words) [view diff] exact match in snippet view article find links to article

D (1993). "Transfection of the malaria parasite and expression of firefly luciferase". Proceedings of the National Academy of Sciences of the United States

Entamoeba (1,482 words) [view diff] exact match in snippet view article find links to article

"Establishment of a transient transfection system and expression of firefly luciferase in Entamoeba invadens". Molecular and Biochemical Parasitology. 183

Brahm Shanker Srivastava (1,101 words) [view diff] exact match in snippet view article find links to article

Srivastava BS (2000). "Bioluminescent Mycobacterium aurum expressing firefly luciferase for rapid and high throughput screening of antimycobacterial drugs

Firefly luciferin (960 words) [view diff] case mismatch in snippet view article find links to article

Gomi K, Kajiyama N (2001). "Oxyluciferin, a Luminescence Product of Firefly Luciferase, Is Enzymatically Regenerated into Luciferin". J Biol Chem. 276 (39):

Viability assay (1,372 words) [view diff] exact match in snippet view article find links to article

viability of cells using the assessment of ATP and a method known as "firefly luciferase". Sodium-potassium ratio: Another kind of assay practices the examination

Protein targeting (6,394 words) [view diff] exact match in snippet view article find links to article

research that led to the discovery of this consensus observed that when firefly luciferase was expressed in cultured insect cells it was targeted to the peroxisome

Paul M. Wassarman (924 words) [view diff] exact match in snippet view article find links to article

"An upstream region of the mouse ZP3 gene directs expression of firefly luciferase specifically to growing oocytes in transgenic mice". Proc Natl Acad

FKBP1A (1,810 words) [view diff] exact match in snippet view article find links to article

"Kinetics of regulated protein-protein interactions revealed with firefly luciferase complementation imaging in cells and living animals". Proceedings

TOC1 (gene) (1,625 words) [view diff] no match in snippet view article

forward genetic screen in which he linked a bioluminescent reporter, firefly (luciferase), to expression of CAB (chlorophyll-a,b binding protein—see Light-harvesting

Massive parallel sequencing (3,019 words) [view diff] exact match in snippet view article find links to article

activity (proof-reading) and luminescence real-time detection using the firefly luciferase. All the key concepts of sequencing by synthesis were introduced,

Long-chain-fatty-acid—CoA ligase (2,740 words) [view diff] exact match in snippet view article find links to article

Conti E, Franks NP, Brick P (March 1996). "Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes". Structure

Firefly (3,946 words) [view diff] exact match in snippet view article find links to article

these substances has been inserted into many different organisms. Firefly luciferase is used in forensics, and the enzyme has medical uses – in particular

Joseph D. Andrade (1,072 words) [view diff] exact match in snippet view article find links to article

reflection fluorescence (TIRF) analysis. The biochemical sensor work used firefly luciferase bioluminescence and enzyme biochemistry for substrate specificity

James Inglese (1,288 words) [view diff] exact match in snippet view article find links to article

"Molecular basis for the high-affinity binding and stabilization of firefly luciferase by PTC124". Proc. Natl. Acad. Sci. USA. 107 (11): 4878–4883. Bibcode:2010PNAS

E-SCREEN (2,108 words) [view diff] exact match in snippet view article find links to article

of a readily measurable protein (the so-called reporter gene; e.g. firefly luciferase). That is an in vitro reporter gene assays detecting estrogen receptor

Norman Sharpless (2,080 words) [view diff] exact match in snippet view article find links to article

'glows' upon activation of the p16INK4a promoter due to insertion of firefly luciferase in place of the endogenous gene. Use of this system revealed the activation

Agrobacterium tumefaciens (4,328 words) [view diff] exact match in snippet view article find links to article

into eukaryotic cells. This process has been performed using the firefly luciferase gene to produce glowing plants. This luminescence has been a useful

Hispid cotton rat (4,532 words) [view diff] exact match in snippet view article find links to article

with a bovine adenovirus type 3 recombinant virus expressing the firefly luciferase gene". Journal of General Virology. 77 (1): 1–9. doi:10.1099/0022-1317-77-1-1

John Woodland Hastings (3,112 words) [view diff] exact match in snippet view article find links to article

in the McElroy lab he examined the basic biochemical mechanism of firefly luciferase and demonstrated that coenzyme A stimulates light emission. His lab

Enzyme inhibitor (11,575 words) [view diff] exact match in snippet view article find links to article

"Molecular basis for the high-affinity binding and stabilization of firefly luciferase by PTC124". Proceedings of the National Academy of Sciences of the

Circadian rhythm (12,903 words) [view diff] exact match in snippet view article find links to article

followed by 3 days of constant light (from 96h). Their genomes carry firefly luciferase reporter genes driven by the promoter sequences of clock genes. The

Photocyte (3,944 words) [view diff] exact match in snippet view article find links to article

PMID 7373026. Keller GA, Gould S, Deluca M, Subramani S (May 1987). "Firefly luciferase is targeted to peroxisomes in mammalian cells". Proceedings of the

Biosensor (12,037 words) [view diff] case mismatch in snippet view article find links to article

Fan, F.; et al. (2008). "Novel Genetically Encoded Biosensors Using Firefly Luciferase". ACS Chem. Biol. 3 (6): 346–51. doi:10.1021/cb8000414. PMID 18570354

MTOR (12,710 words) [view diff] exact match in snippet view article find links to article

"Kinetics of regulated protein-protein interactions revealed with firefly luciferase complementation imaging in cells and living animals". Proceedings

Theories of general anaesthetic action (6,745 words) [view diff] exact match in snippet view article find links to article

Lieb WR, Brick P (1998). "Structural basis for the inhibition of firefly luciferase by a general anesthetic". Biophysical Journal. 75 (5): 2205–2211.

List of EC numbers (EC 1) (24,607 words) [view diff] exact match in snippet view article

luciferase EC 1.13.12.6: Cypridina-luciferin 2-monooxygenase EC 1.13.12.7: firefly luciferase EC 1.13.12.8: Watasenia-luciferin 2-monooxygenase EC 1.13.12.9: phenylalanine

Ravi Allada (4,524 words) [view diff] exact match in snippet view article find links to article

Allada and his lab team administered luciferin, a substrate for firefly luciferase reporters and discovered evidence of a functional role in Drosophila

Thermal shift assay (5,530 words) [view diff] exact match in snippet view article find links to article

"Molecular basis for the high-affinity binding and stabilization of firefly luciferase by PTC124". Proceedings of the National Academy of Sciences of the

Jay Dunlap (5,405 words) [view diff] exact match in snippet view article find links to article

Dunlap, along with Jennifer Loros, Arun Mehra, and Van Gooch, adapted firefly luciferase for expression in Neurospora, thereby greatly expanding the ability

Stress granule (12,548 words) [view diff] exact match in snippet view article find links to article

"Stable transformation of an Arabidopsis cell suspension culture with firefly luciferase providing a cellular system for analysis of chaperone activity in

Murine respirovirus (31,229 words) [view diff] exact match in snippet view article find links to article

"Creation of an infectious recombinant Sendai virus expressing the firefly luciferase gene from the 3' proximal first locus". The Journal of General Virology